The following chemicals and drugs were used: acetylcholine chloride (Sigma-Aldrich, St. Louis, MO, United States), acetonitrile (ACN, Merck, Darmstadt, Germany), ammonium bicarbonate (Sigma-Aldrich, St. Louis, MO, United States), carbamylcholine chloride (carbachol; Sigma-Aldrich, St. Louis, MO, United States), dithiothretiol (Merck, Darmstadt, Germany), formic acid (Sigma-Aldrich, St. Louis, MO, United States), iodoacetamide (GE Healthcare, Uppsala, Sweden), LCMS grade acetonitrile (Fisher Scientific, Loughborough, United Kingdom), potassium chloride (KCl, Ajax Finechem Pty. Ltd., Taren Point, Australia), proteomics grade bovine trypsin (Sigma-Aldrich, St. Louis, MO, United States), trifluoroacetic acid (TFA, Auspep, Melbourne, Australia), d -tubocurarine (Sigma-Aldrich, St. Louis, MO, United States) and trifluroethanol (Sigma-Aldrich, St. Louis, MO, United States). Unless otherwise indicated, all chemicals were dissolved or diluted in milli-Q water.
Proteomics grade bovine trypsin
Manufactured by Merck Group
Sourced in Australia
Proteomics grade bovine trypsin is a highly purified enzyme derived from bovine pancreas. It is commonly used in proteomic applications for the digestion of proteins into smaller peptides, which can then be analyzed using mass spectrometry techniques.
Automatically generated - may contain errors
Lab products found in correlation
D tubocurarine, by Merck Group (3 mentions)
Lc ms grade acetonitrile, by Thermo Fisher Scientific (3 mentions)
Ammonium bicarbonate, by Merck Group (3 mentions)
Acetonitrile acn, by Merck Group (3 mentions)
Acetylcholine chloride, by Merck Group (3 mentions)
Iodoacetamide, by GE Healthcare (3 mentions)
Formic acid, by Merck Group (3 mentions)
Dithiothretiol, by Merck Group (3 mentions)
Trifluroethanol, by Merck Group (3 mentions)
Carbamylcholine chloride carbachol, by Merck Group (3 mentions)
3 protocols using proteomics grade bovine trypsin
1
Acetylcholine Receptor Activation Protocol
2
Mass Spectrometry Analysis of Neurotransmitters
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The following chemicals and drugs were used: acetylcholine chloride (Sigma-Aldrich, St. Louis, MO, United States), acetic acid sodium salt (sodium acetate, Merck, Darmstadt, Germany), acetonitrile (ACN, Merck, Darmstadt, Germany), ammonium bicarbonate (Sigma-Aldrich, St. Louis, MO, United States), carbamylcholine chloride (carbachol; Sigma-Aldrich, St. Louis, MO, United States), dithiothretiol (Merck, Darmstadt, Germany), formic acid (Sigma-Aldrich, St. Louis, MO, United States), iodoacetamide (GE Healthcare, Uppsala, Sweden), LCMS grade acetonitrile (Fisher Scientific, Loughborough, United Kingdom), potassium chloride (KCl, Ajax Finechem Pty. Ltd., Taren Point, Australia), proteomics grade bovine trypsin (Sigma-Aldrich, St. Louis, MO, United States), trifluoroacetic acid (TFA, Auspep, Melbourne, Australia), d-tubocurarine (Sigma-Aldrich, St. Louis, MO, United States) and trifluroethanol (Sigma-Aldrich, St. Louis, MO, United States). Unless otherwise indicated, all chemicals were dissolved or diluted in milli-Q water.
3
Proteomic Analysis of Venom Neurotoxins
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The following chemicals and drugs were used: acetylcholine chloride (Sigma-Aldrich, St. Louis, MO, USA), acetonitrile (ACN, Merck, Darmstadt, Germany), ammonium bicarbonate (Sigma-Aldrich), carbamylcholine chloride (carbachol; Sigma-Aldrich), dithiothretiol (Merck), formic acid (Sigma-Aldrich), iodoacetamide (GE Healthcare, Uppsala, Sweden), LCMS grade acetonitrile (Fisher Scientific, Loughborough, UK), potassium chloride (KCl, Ajax Finechem Pty. Ltd., Taren Point, Australia), proteomics grade bovine trypsin (Sigma-Aldrich), taipan antivenom (CSL Ltd., Melbourne, Australia), trifluoroacetic acid (TFA, Auspep, Melbourne, Australia), d-tubocurarine (Sigma-Aldrich) and trifluroethanol (Sigma-Aldrich). All chemicals were dissolved or diluted in MilliQ water for unless otherwise stated.
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