Hep3B and HepG2 cell lines were purchased from the Leibniz-Institut DSMZ, Braunschweig (ACC-93, ACC-180) and grown in DMEM (Euroclone, ECM0749L) and RPM1640 (Euroclone, ECB9006L), respectively, supplemented with 10% FBS (Euroclone, ECS0180L) and 1% penicillin-streptomycin. HuS cells were a gift of Dr. Vinicio Carloni, University of Florence and were grown in DMEM containing 4.5 g/L of glucose (Euroclone, ECB7501L) and supplemented with 10% FBS (Euroclone, ECS0180L), 5 ng/mL EGF (Sigma-Aldrich, E9644), 420 ng/mL insulin (Sigma-Aldrich, I9278), 20 ng/mL selenium (Sigma-Aldrich, S9133), 1% DMSO (Amresco, 0231) and 1% penicillin-streptomycin (Euroclone, ECB3001D). Cells have been authenticated by STR PCR by the supplier and routinely tested for Mycoplasma contamination using Myco Alert (Lonza, LT07-318).
Ecs0180l
Manufactured by Merck Group
The ECS0180L is a laboratory equipment product manufactured by Merck Group. It serves as a core function within the research and development process, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using ecs0180l
1
Cell Line Cultivation and Authentication
2
Breast Cancer Cell Lines and Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
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MCF7, ZR75.1 and HCC1428 human female breast cancer cells were obtained from ATCC and cultured in phenol red-free RPMI 1640 medium (GIBCO #11835-063) supplemented with 10% fetal bovine serum (FBS, Euroclone #ECS0180L) 2 mmol/L glutamine (Sigma #G7513) and 1 nmol/L 17β-estradiol (E2, Sigma #E2758). The corresponding LTED (long term estrogen deprived) derivatives were maintained in sterol-deprived medium in phenol red–free RPMI1640 medium containing 10% dextran charcoal-stripped FBS (Hyclone #SH30068.03) and 2 mmol/L glutamine (DCC medium). Tamoxifen- (TAMR) and fulvestrant- (FULVR) resistant cells were cultured in DCC medium supplemented with 1 μmol/L 4-OH tamoxifen (Sigma #H7904) or 100 nmol/L fulvestrant (Sigma #I4409), respectively. Cells were short tandem repeat tested, amplified, stocked, routinely subjected to mycoplasma testing and once thawed were kept in culture for a maximum of 20 passages.
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