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Jms t100lp accutof lc plus 4 g mass spectrometer

Manufactured by JEOL

The JMS-T100LP AccuTOF LC-plus 4 G is a high-resolution time-of-flight mass spectrometer designed for liquid chromatography applications. It provides accurate mass measurements and high mass resolution for the identification and characterization of unknown compounds.

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2 protocols using jms t100lp accutof lc plus 4 g mass spectrometer

1

Mass Spectrometry Analysis of RNA-NCD Complex

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Samples were prepared by mixing UGGAA/UGGAA pentad-containing hairpin-RNA (10 µM) and NCD (5–40 µM) in water containing 100 mM ammonium acetate and 50% methanol. Mass spectra were obtained with JEOL JMS-T100LP AccuTOF LC-plus 4 G mass spectrometer in negative mode. Spray temperature was fixed at –10 °C.
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2

CSI-TOF MS Analysis of RNA Duplex

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Time-of-flight mass spectrometry analysis by cold spray ionization (CSI-TOF MS) data were measured on JEOL JMS-T100LP AccuTOF LC-plus 4G mass spectrometer in negative mode. Samples containing RNA duplex r(C/CC) [r(C): 5′-r(GUC CAG C GCA ACG)-3′ / r(CC): 3′-r(CAG GUC CC CGU UGC)-5′] (10 μM) with or without ANP77 (50 μM) in 1:1 mixture of water and methanol containing ammonium acetate (50 mM) were sprayed at a flow rate of 14 μl min–1. Spray temperature was fixed at –10°C during the injection.
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