Bfa powder

Manufactured by Merck Group

BFA powder is a laboratory reagent produced by Merck Group. It is a white, crystalline powder used as a component in various analytical and research applications. The core function of BFA powder is to serve as a chemical intermediate or building block in the synthesis of other compounds. No further details on its intended use or applications are provided.

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Lab products found in correlation

Fm1 43 dye, by Thermo Fisher Scientific (2 mentions)

2 protocols using bfa powder

Visualizing Arabidopsis Root Dynamics

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For BFA treatment, a stock solution of 10 mM was made by dissolving BFA powder (Sigma-Aldrich) in DMSO. Subsequently, the working solution of 50 µM was diluted in 0.5× MS, 1% sucrose solution, and loaded into a 1-mL syringe. The BFA solution was injected directly next to the roots of 4- to 5-d-old Arabidopsis seedlings grown on coverslips and incubated at room temperature for 10 min prior to microscopy.
FM 1-43 dye (ThermoFisher Scientific) was dissolved in DMSO to make a 10-mM stock solution. The dye was diluted to 4 µM in 0.5× MS, 1% sucrose solution. The dye was loaded into a 1-mL syringe, and the solution was injected next to the roots as described above.

Chin S., Kwon T., Khan B.R., Sparks J.A., Mallery E.L., Szymanski D.B, & Blancaflor E.B. (2021). Spatial and temporal localization of SPIRRIG and WAVE/SCAR reveal roles for these proteins in actin-mediated root hair development. The Plant Cell, 33(7), 2131-2148.

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Visualizing Arabidopsis Root Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

For BFA treatment, a stock solution of 10 mM was made by dissolving BFA powder (Sigma-Aldrich) in DMSO. Subsequently, the working solution of 50 µM was diluted in 0.5 x MS, 1% sucrose solution and loaded into a 1 mL syringe. The BFA solution was injected directly next to the roots of 4-5 days old Arabidopsis seedlings grown on coverslips and was incubated in room temperature for 10 minutes prior to microscopy. FM 1-43 dye (ThermoFisher Scientific) was dissolved in DMSO to make a 10 mM stock solution. The dye was diluted to 4 µM in 0.5 x MS, 1% sucrose solution. The dye was loaded into a 1 mL syringe and the solution was injected next to the roots as described above.

Chin S., Kwon T., Khan B.R., Sparks J.A., Mallery E.L., Szymanski D.B., & Blancaflor E.B. (2020). Spatial and Temporal Localization of SPIRRIG and WAVE/SCAR Reveal Roles for These Proteins in Actin-Mediated Root Hair Development.

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