Western blot analysis was performed as described previously (24 (link)). The following antibodies were used: mouse monoclonal anti-p53 (DO-1; Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit polyclonal anti-XPO1 (Santa Cruz Biotechnology); rabbit polyclonal anti-HSP-90a/b (Santa Cruz Biotechnology); rabbit monoclonal histone H3 (Cell Signaling Technologies, Beverly, MA, USA); rabbit monoclonal GAPDH (Cell Signaling Technologies); and mouse monoclonal anti-β-actin (Sigma Chemical Co., St Louis, MO, USA). Nuclear and cytoplasmic proteins were extracted using a subcellular fractionation kit (ProteoExtract; EMD Millipore Corporation, Billerica, MA, USA), according to the manufacturer's protocol. Protein lysates were also subjected to immunoprecipitation using anti-XPO1 and immunoprecipitates were subjected to western blot analysis with anti-XPO1 or p53. Visualized blots were analyzed by the MultiGauge 3.1 software (Fujifilm, Tokyo, Japan).
Rabbit polyclonal anti hsp 90a b
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Rabbit polyclonal anti-HSP-90α/β is a laboratory reagent used to detect and quantify the expression of the Heat Shock Protein 90 (HSP-90) in biological samples. HSP-90 is a highly conserved molecular chaperone that plays a crucial role in the folding, stability, and function of various client proteins.
Automatically generated - may contain errors
Lab products found in correlation
Multi gauge 3, by Fujifilm (2 mentions)
Mouse monoclonal anti p53 do 1, by Santa Cruz Biotechnology (2 mentions)
Rabbit polyclonal anti xpo1, by Santa Cruz Biotechnology (2 mentions)
Rabbit monoclonal gapdh, by Cell Signaling Technology (2 mentions)
Rabbit monoclonal histone h3, by Cell Signaling Technology (2 mentions)
Proteoextract, by Merck Group (2 mentions)
Mouse monoclonal anti β actin, by Merck Group (2 mentions)
2 protocols using rabbit polyclonal anti hsp 90a b
1
Analysis of XPO1-p53 Interaction by Western Blot
2
Western Blot Analysis of Protein Localization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Western blot analysis was carried out as described previously.(24 (link)) The following antibodies were used: mouse monoclonal anti-p53 (DO-1; Santa Cruz Biotechnology, Santa Cruz, CA, USA); rabbit polyclonal anti-XPO1 (Santa Cruz Biotechnology); rabbit polyclonal anti-HSP-90a/b (Santa Cruz Biotechnology); rabbit monoclonal histone H3 (Cell Signaling Technologies, Beverly, MA, USA); rabbit monoclonal GAPDH (Cell Signaling Technologies); and mouse monoclonal anti-β-actin (Sigma Chemical Co., St Louis, MO, USA). Nuclear and cytoplasmic proteins were extracted using a subcellular fractionation kit (ProteoExtract; EMD Millipore, Billerica, MA, USA), according to the manufacturer's protocol. Protein lysates were also subjected to immunoprecipitation using anti-XPO1 and immunoprecipitates were subjected to Western blot analysis with anti-XPO1 or p53. Visualized blots were analyzed by the MultiGauge 3.1 software (Fujifilm, Tokyo, Japan).
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