Engineered tissues and host auxiliary tissues were harvested and formalin-fixed, paraffin-embedded for histology. Embedded samples were sectioned, and heat-induced antigen retrieval was performed in a pressure cooker with sodium citrate buffer. Samples were blocked with 1.5% normal goat serum, followed by incubation with primary antibodies listed in Table 1 overnight at 4°C, and incubation with secondary antibodies for 1 hour at room temperature or overnight at 4°C. Secondary antibodies used were donkey anti-goat Alexa Fluor 488 (Invitrogen), donkey anti-goat IgG Alexa Fluor 488 conjugate (Invitrogen), donkey anti-mouse Alexa Fluor® 555 conjugate (Invitrogen), donkey anti-rabbit IgG Alexa Fluor 594 conjugate (Novex), and goat anti-mouse IgG1 Alexa Fluor 555 conjugate (Novex). Nuclei were counterstained with Hoechst or DAPI. Immunohistochemical detection of human mitochondria was performed using the mouse-specific HRP/DAB (ABC) Detection IHC Kit (ab64259). Images were acquired using the microscopes specified in the following section.
Donkey anti mouse alexa fluor 555 conjugate
Manufactured by Thermo Fisher Scientific
The Donkey anti-mouse Alexa Fluor® 555 conjugate is a secondary antibody that binds to mouse primary antibodies. The Alexa Fluor® 555 dye conjugated to the antibody emits fluorescent signal in the orange-red spectrum when excited, allowing for visualization and detection of target molecules in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Alexa fluor 488 donkey anti goat, by Thermo Fisher Scientific (2 mentions)
Donkey anti goat igg alexa fluor 488 conjugate, by Thermo Fisher Scientific (2 mentions)
Donkey anti rabbit igg alexa fluor 594 conjugate, by Thermo Fisher Scientific (2 mentions)
Goat anti mouse igg1 alexa fluor 555 conjugate, by Thermo Fisher Scientific (2 mentions)
2 protocols using donkey anti mouse alexa fluor 555 conjugate
1
Immunohistochemical Analysis of Engineered Tissues
2
Immunohistochemical Analysis of Engineered Tissues
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Engineered tissues and host auxiliary tissues were harvested and formalin-fixed, paraffin-embedded for histology. Embedded samples were sectioned, and heat-induced antigen retrieval was performed in a pressure cooker with sodium citrate buffer. Samples were blocked with 1.5% normal goat serum, followed by incubation with primary antibodies listed in Table 1 overnight at 4°C, and incubation with secondary antibodies for 1 hour at room temperature or overnight at 4°C. Secondary antibodies used were donkey anti-goat Alexa Fluor 488 (Invitrogen), donkey anti-goat IgG Alexa Fluor 488 conjugate (Invitrogen), donkey anti-mouse Alexa Fluor® 555 conjugate (Invitrogen), donkey anti-rabbit IgG Alexa Fluor 594 conjugate (Novex), and goat anti-mouse IgG1 Alexa Fluor 555 conjugate (Novex). Nuclei were counterstained with Hoechst or DAPI. Immunohistochemical detection of human mitochondria was performed using the mouse-specific HRP/DAB (ABC) Detection IHC Kit (ab64259). Images were acquired using the microscopes specified in the following section.
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