For production of spray dried LNPs, a B-290 spray drying tower (Büchi Labortechnik, Flawil, Schweiz) was used. Pumpsil Tubing 1.6 mm x 1.6 mm (Watson Marlow Tubing, Falmouth, UK) with a pump rate of 1.4 mL/min was chosen. Nitrogen functioned as atomizing gas, whereas drying gas was air. In order to avoid dust and other airborne particles, both nitrogen gas and air were filtered through a 0.2 μm PTFE membrane pore. Pressurized air was used to ensure sufficient heating of the air supply and to avoid overheating of the Büchi’s vacuum pump. The aspirator was set to 70% and vacuum ranged from -40 mbar to -35 mbar. The airflow was set to 40 mm corresponding to 473 NL/h. The inlet-temperatures (T-In) were set to 100 °C and 120 °C resulting in measured outlet-temperatures (T-Out) of accordingly 62 °C and 72 °C ± 2 °C, respectively. Each individual stock solution of LNP-siRNA formulations was diluted to a concentration of 30 μg siRNA in 5000 μL of a specified solvent (highly purified water (HPW) with lactose (InhaLac) at 5% w/V, sterile filtered). This resulted in an siRNA to sugar concentration of 0.12 μg siRNA/mg lactose.
B 290 spray drying tower
Manufactured by Büchi
The B-290 spray drying tower is a laboratory-scale equipment designed for the drying of liquid samples. It features a drying chamber, an air inlet, and a cyclone separator to collect the dried material. The core function of the B-290 is to transform liquid samples into dry powders through the process of spray drying.
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2 protocols using b 290 spray drying tower
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Spray Dried Lipid Nanoparticle Production
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Spray Dried Lipid Nanoparticle Production
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For production of spray dried LNPs, a B-290 spray drying tower (Büchi Labortechnik, Flawil, Schweiz) was used. Pumpsil Tubing 1.6 mm x 1.6 mm (Watson Marlow Tubing, Falmouth, UK) with a pump rate of 1.4 mL/min was chosen. Nitrogen functioned as atomizing gas, whereas drying gas was air. In order to avoid dust and other airborne particles, both nitrogen gas and air were filtered through a 0.2 μm PTFE membrane pore. Pressurized air was used to ensure sufficient heating of the air supply and to avoid overheating of the Büchi’s vacuum pump. The aspirator was set to 70% and vacuum ranged from -40 mbar to -35 mbar. The airflow was set to 40 mm corresponding to 473 NL/h. The inlet-temperatures (T-In) were set to 100 °C and 120 °C resulting in measured outlet-temperatures (T-Out) of accordingly 62 °C and 72 °C ± 2 °C, respectively. Each individual stock solution of LNP-siRNA formulations was diluted to a concentration of 30 μg siRNA in 5000 μL of a specified solvent (highly purified water (HPW) with lactose (InhaLac) at 5% w/V, sterile filtered). This resulted in an siRNA to sugar concentration of 0.12 μg siRNA/mg lactose.
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