Cell-extract proteins were resolved by Tris-Acetate PAGE and Bis-Tris gels (Invitrogen, Carlsbad, CA, USA) and transferred onto polyvinylidene difluoride membranes (PVDF), blocked in 5% milk in PBST, and incubated overnight with indicated antibodies. Membranes were washed and incubated with HRP-conjugated antirabbit IgG or antimouse IgG (Cell Signaling Technology, Danvers, MA, USA), followed by detection using BioRad Gel Doc. For Coomassie staining, proteins were resolved on PAGE gels (Invitrogen, Carlsbad, CA, USA) followed fixation and staining with Bio-Safe Coomassie G-250 stain (Bio-Rad Laboratories, Hercules, CA, USA) according to manufacturer’s instructions.
Tris acetate page and bis tris gels
Manufactured by Thermo Fisher Scientific
Sourced in United States
Tris-Acetate PAGE and Bis-Tris gels are laboratory equipment used for the separation and analysis of proteins. These gels facilitate the electrophoretic separation of proteins based on their molecular weight and charge. The Tris-Acetate PAGE gel system utilizes a Tris-Acetate buffer, while the Bis-Tris gel system employs a Bis-Tris buffer. Both systems provide reliable and reproducible protein separation for various applications in biochemistry and molecular biology.
Automatically generated - may contain errors
2 protocols using tris acetate page and bis tris gels
1
Immunoblotting with Tris-Acetate and Bis-Tris Gels
2
Protein Separation and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell extract proteins were resolved by Tris-Acetate PAGE and Bis-Tris gels (Invitrogen, Carlsbad, CA, USA) and transferred onto polyvinylidene difluoride membranes, which were incubated overnight with primary antibodies, washed, and incubated with HRP-conjugated anti-rabbit Ig or anti-mouse Ig (Amersham Biosciences, Little Chalfont, UK) for 1 h.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!