Four brown seaweeds were obtained from several local markets: S. horneri from Kouda Co., Ltd. (Sado, Japan), C. okamuranus from Kaiso-tonya (Okinawa, Japan), N. decipiens from Kawashimaya (Niigata, Japan), and K. crassifolia from Hakodate-Kaisoya (Hakodate, Japan). These brown seaweeds were harvested at the sea near the district of the market. Crude fucoidan from F. vesiculosus was purchased from Sigma-Aldrich Co., (St. Louis, USA) and was purified from the second step of the purification procedure described below.
Crude fucoidan from f vesiculosus
Manufactured by Merck Group
Sourced in United States
Crude fucoidan from F. vesiculosus is a natural compound extracted from the brown seaweed Fucus vesiculosus. It is a sulfated polysaccharide with a core function as a structural component in the cell walls of the seaweed.
Automatically generated - may contain errors
Lab products found in correlation
Anti rabbit igg hrp linked antibody, by Cell Signaling Technology (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Poly adp ribose polymerase parp, by Cell Signaling Technology (1 mentions)
4.5 g l glucose, by Thermo Fisher Scientific (1 mentions)
L glutamine and sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Mouse monoclonal β actin antibody, by Merck Group (1 mentions)
Cleaved parp d214, by Cell Signaling Technology (1 mentions)
Phospho beclin 1 ser15, by Cell Signaling Technology (1 mentions)
2 protocols using crude fucoidan from f vesiculosus
1
Extraction and Purification of Fucoidan
2
Autophagy Signaling Pathway Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies to Akt, phospho-Akt (Ser473), phospho-Akt (Thr308), beclin-1, phospho-Beclin-1 (Ser15), phospho-PI3Kinase p85(Tyr458)/p55 (Tyr199), phospho-4E-BP1 (Thr37/46), p70S6K, phospho-p70S6K(Thr389), LC3B, poly(ADP-ribose) polymerase (PARP), cleaved PARP (D214) and anti-rabbit IgG HRP-linked antibody were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal β-Actin antibody and crude fucoidan from F. vesiculosus were obtained from Sigma-Aldrich (St Louis, MO, USA). DMEM (Dulbecco’s Modification of Eagle’s Medium) with 4.5 g/L glucose, L-glutamine and sodium pyruvate, trypsin-EDTA, penicillin-streptomycin-amphotericin B solution (50X), fetal bovine serum (FBS), phosphate buffer solution (PBS), and PBS with Tween 20 (PBST) were purchased from Life Technologies (Waltham, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!