Ck14 antibody

Manufactured by Santa Cruz Biotechnology

The CK14 antibody is a laboratory tool used to detect the presence of cytokeratin 14 protein in biological samples. Cytokeratin 14 is a structural protein found in certain cell types, and its identification can provide information about the characteristics of the cells being studied.

Automatically generated - may contain errors

Lab products found in correlation

Igf1 r antibody, by Abcam (1 mentions) Anti pan cytokeratin, by Agilent Technologies (1 mentions)

2 protocols using ck14 antibody

Identifying Cell Surface Markers in DFCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To identify cell surface markers of DFCs, iDFCs and dDFCs, cells were first transferred into a 24-well plate and cultured for 14 hours. Cells were then fixed with 4% polyoxymethylene(PFA) for 30 min, followed by perforation with 0.5% TritonX-100 for 20 min, and block with 10% BSA for 1 hour. First antibodies used were vimentin antibody (ImmunoWay, YT4880, 1:2000) and CK14 antibody (Santa Cruz Biotechnology, SC23878, 1:1000). After incubation with first antibody for 14hours, cells were rinsed with PBS and then incubated with DyLight 488 AffiniPure Goat AntiRabbit IgG or DyLight 488 AffiniPure Goat Anti-Mouse IgG respectively for half an hour. Cell nuclei were stained with 100 ng/ml DAPI for 5 min. Then photos of stained cells were taken under a confocal laser scanning microscopy.

Wu Y., Feng G., Song J., Zhang Y., Yu Y., Huang L., Zheng L, & Deng F. (2015). TrAmplification of Human Dental Follicle Cells by piggyBac Transposon - Mediated Reversible Immortalization System. PLoS ONE, 10(7), e0130937.

+ Open protocol

+ Expand

Immunohistochemistry Analysis of FOXN1, IGF-1R, and Cytokeratins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed paraffin embedded samples and frozen samples were used for immunohistochemistry. Anti-Forkhead box protein N1 (FOXN1) antibody (Santa Cruz: sc-23566), anti-insulin growth factor 1 receptor (IGF-1R) antibody (Abcam: ab90657), anti-pan cytokeratin (Dako), and anti-cytokeratin 14 (CK14) antibody (Santa Cruz: sc-53253) were used for immunohistochemistry, which was performed as previously described (14 (link)). The number of FOXN1 positive cells in the thymus or thymic grafts was determined by counting the number of positive cells in at least five randomly chosen high power fields (x400).

Tasaki M., Villani V., Shimizu A., Sekijima M., Yamada R., Hanekamp I.M., Hanekamp J.S., Cormack T.A., Moran S.G., Kawai A., Sachs D.H, & Yamada K. (2016). Role of bone marrow maturity, insulin-like growth factor 1 receptor and forkhead box protein N1 in thymic involution and rejuvenation. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(10), 2877-2891.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!