Il 2 elisa kit

Manufactured by Thermo Fisher Scientific

Sourced in United States

The IL-2 ELISA kit is a laboratory assay used to quantify the levels of interleukin-2 (IL-2) in biological samples. IL-2 is a cytokine that plays a crucial role in the activation and proliferation of T cells. The kit employs the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of IL-2 in the sample.

Automatically generated - may contain errors

Lab products found in correlation

Tnf α elisa kit, by Thermo Fisher Scientific (1 mentions) Human xl cytokine array, by R&D Systems (1 mentions) Anti cd3 14 0037 82, by Thermo Fisher Scientific (1 mentions) Ifn γ elisa kit, by Thermo Fisher Scientific (1 mentions) Anti cd3, by Thermo Fisher Scientific (1 mentions) Anti cd28, by Thermo Fisher Scientific (1 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Flat bottomed plates, by Thermo Fisher Scientific (1 mentions) Microplate reader, by Bio-Rad (1 mentions) Jem 1011, by JEOL (1 mentions) Av 300 nmr spectrometer, by Bruker (1 mentions) 3 aminopropyltriethoxysilane, by Merck Group (1 mentions) Mda mb 231 cell, by American Type Culture Collection (1 mentions) Cell culture media and reagents, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate pma ionomycin, by Merck Group (1 mentions)

Spelling variants of this product

ELISAs (35 citations)

11 protocols using il 2 elisa kit

Vitamin C Enhances T-Cell IL-2 Production

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human T cells (5×10⁵/well) were cultured in a 24-well plate
and activated with PMA/ionomycin. Vitamin C was added 2 hours before or 24 hours after activation at a concentration of 500 mM. Culture sup was obtained 36 hours after activation and enzyme-linked immunosorbent analysis (ELISA) for IL-2 was performed using IL-2 ELISA kit (Invitrogen) following the manufacturer's instruction.

Hong J.M., Kim J.H., Kang J.S., Lee W.J, & Hwang Y.I. (2016). Vitamin C is taken up by human T cells via sodium-dependent vitamin C transporter 2 (SVCT2) and exerts inhibitory effects on the activation of these cells in vitro. Anatomy & Cell Biology, 49(2), 88-98.

+ Open protocol

+ Expand

Profiling Cytokine Production in Resting T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess cytokine and chemokine production, non-activated T cells (not treated with CD3/28 stimulator or IL-2) were used. For preparing the antibody pre-coated plate, the following antibodies with indicated concentrations were used: anti-CD3 (14-0037-82, Invitrogen; 0.5 μg/mL), anti-CD81 (349502, BioLegend; 5 μg/mL), and anti-CD82 (342102, BioLegend; 5 μg/mL). These antibodies were resuspended in DPBS and coated onto the plate at 4°C, 24 hours before use. For RNA sequencing (RNA-seq), cells were harvested on Day 1. For cytokine analysis and flow cytometry, the cells were harvested on Day 2. To determine the concentrations of IFN-γ, TNF-α, and IL-2 in the T-cell supernatant, we used the following commercially available ELISA kits: Human IFN-γ ELISA kit (EHIFNG, Invitrogen), TNF-α ELISA kit (KHC3011, Invitrogen), and IL-2 ELISA kit (BMS221, Invitrogen). Additionally, we used the Human XL Cytokine Array (ARY022B, R&D Systems) to assess the concentration of various cytokines in the T-cell supernatant after they were cultured for either 2 or 3 days.

Na K., Lee S., Kim D.K., Kim Y.S., Hwang J.Y., Kang S.S., Baek S., Lee C.Y., Yang S.M., Han Y.J., Kim M.H., Han H., Kim Y., Kim J.H., Jeon S., Byeon Y., Lee J.B., Lim S.M., Hong M.H., Pyo K.H, & Cho B.C. (2024). CD81 and CD82 expressing tumor-infiltrating lymphocytes in the NSCLC tumor microenvironment play a crucial role in T-cell activation and cytokine production. Frontiers in Immunology, 15, 1336246.

+ Open protocol

+ Expand

Quantifying Serum IL-2 and IL-15

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum was analyzed for IL-2 using an IL-2 ELISA kit (Invitrogen) and for IL-15 using IL-15 High Performance Luminex kit (R&D Systems).

Zhang Z., Gothe F., Pennamen P., James J.R., McDonald D., Mata C.P., Modis Y., Alazami A.M., Acres M., Haller W., Bowen C., Döffinger R., Sinclair J., Brothers S., Zhang Y., Matthews H.F., Naudion S., Pelluard F., Alajlan H., Yamazaki Y., Notarangelo L.D., Thaventhiran J.E., Engelhardt K.R., Al-Mousa H., Hambleton S., Rooryck C., Smith K.G, & Lenardo M.J. (2019). Human interleukin-2 receptor β mutations associated with defects in immunity and peripheral tolerance. The Journal of Experimental Medicine, 216(6), 1311-1327.

+ Open protocol

+ Expand

Cytokine Quantification by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols

IL-2 and IFN-γ measurements in the cytotoxicity assay supernatants were analyzed using ELISA per the conditions provided by IFN-γ ELISA kit (Invitrogen) and IL-2 ELISA kit (Invitrogen). In brief, following the incubation period the supernatant was removed and 10-fold diluted into ELISA buffer (provided in the kit) prior to placing 50 μL into the respective well of the included ELISA 96-well plate. IFN-γ and IL-2 production of experimental wells was determined through a standard curve generated from known control sample concentrations.

Petersburg J., Vallera D.A, & Wagner C.R. (2023). ERADICATION OF HETEROGENEOUS TUMORS BY T-CELLS TARGETED WITH COMBINATION BISPECIFIC CHEMICALLY SELF-ASSEMBLED NANORINGS (CSANs). Molecular cancer therapeutics, 22(3), 371-380.

+ Open protocol

+ Expand

Jurkat T Cell IL-2 Secretion Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Jurkat T cells were co-stimulated with antibodies against CD3 and CD28 (Peprotech, Rocky Hill, NJ, USA) to induce the secretion of IL-2. Briefly, 5 μg/ml anti-CD3 was added to a 96-well plate at 50 μl/well, which was then incubated for 3 h at 37°C and then washed three times with DPBS. Jurkat T cells were seeded at a density of 5 × 10⁵ cells/well. Then, 2 μg/ml anti-CD28 per well (1 μl) was added to the wells, and the plate was incubated for 72 h at 37°C and 5% CO₂. The culture supernatant was then collected and diluted 1:3, after which the total amount of IL-2 secreted by Jurkat T cells was measured using an IL-2 ELISA kit (Peprotech) per manufacturer’s instructions.

Kim H.J., Nam Y.R., Woo J., Kim W.K, & Nam J.H. (2020). Gardenia jasminoides extract and its constituent, genipin, inhibit activation of CD3/CD28 co-stimulated CD4+ T cells via ORAI1 channel. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 24(4), 363-372.

+ Open protocol

+ Expand

Induce IL-2 Secretion in Jurkat T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Jurkat T cells were stimulated with anti-CD3 (Peprotech, Rocky Hill, NJ) and anti-CD28 (Peprotech) to induce the secretion of interleukin-2 (IL-2). Briefly, 50 µL/well of anti-CD3 at a concentration of 5 µg/mL was added to a 96-well plate, incubated at 37 °C for 3 h, and washed three times with Dulbecco’s phosphate-buffered saline (DPBS). Jurkat T cells were seeded at a density of 5 × 10⁵ cells/well. Thereafter 2 µg/mL anti-CD28 was added into each well and cultured in a 5% CO₂ incubator at 37 °C for 72 h. The culture solution was subsequently collected and diluted 1:3 with DMEM. The total amount of IL-2 secreted by Jurkat T cells was measured using the IL-2 ELISA kit (Peprotech) according to the manufacturer’s protocol.

Kim H.J., Park S., Shin H.Y., Nam Y.R., Lam Hong P.T., Chin Y.W., Nam J.H, & Kim W.K. (2021). Inhibitory effects of α-Mangostin on T cell cytokine secretion via ORAI1 calcium channel and K+ channels inhibition. PeerJ, 9, e10973.

+ Open protocol

+ Expand

IL-2 Production by Activated T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

A total of 1 × 10⁵ sorted CD4⁺ Foxp3^- CD44^lo T cells were added to a 96-well plate with either wells precoated with anti-CD3ε and anti-CD28 or empty wells. After 24 h, the cells were centrifuged to collect the supernatant. An IL-2 ELISA kit (eBiosciences, California, USA) was used to measure the amount of IL-2 in the supernatant.

Prasad M., Brzostek J., Gautam N., Balyan R., Rybakin V, & Gascoigne N.R. (2020). Themis regulates metabolic signaling and effector functions in CD4+ T cells by controlling NFAT nuclear translocation. Cellular and Molecular Immunology, 18(9), 2249-2261.

+ Open protocol

+ Expand

CD4+ T Cell Activation Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

To stimulate CD4⁺ T cells, we coated 96-well flat-bottomed plates (Nunc) with anti-CD3ε (2.5 μg/mL) by overnight incubation at 4 °C. CD4⁺ T cells were negatively isolated with a CD4⁺ T cell isolation kit (Miltenyi Biotec, Bergisch-Gladbach, Germany) in accordance with the manufacturer’s instructions. CD4⁺ T cell purity was determined by flow cytometry (CD3⁺, CD4⁺ cells >90 % purification). Immediately after cell preparation, CD4⁺ T cells were added to the coating plate (1 × 10⁵ cells/well). C₆₀-P, C₆₀(OH)₃₆, or NAC was added to the well 30 min before the addition of anti-CD28 (1 μg/mL). After incubation of the cells for 3 days at 37 °C (95 % room air, 5 % CO₂), the amount of IL-2 released into an aliquot of culture supernatant was measured with an IL-2 ELISA kit (eBioscience) in accordance with the manufacturer’s instructions.

Hirai T., Yoshioka Y., Udaka A., Uemura E., Ohe T., Aoshima H., Gao J.Q., Kokubo K., Oshima T., Nagano K., Higashisaka K., Mashino T, & Tsutsumi Y. (2016). Potential Suppressive Effects of Two C60 Fullerene Derivatives on Acquired Immunity. Nanoscale Research Letters, 11, 449.

+ Open protocol

+ Expand

IL2 Quantification in Coculture

Check if the same lab product or an alternative is used in the 5 most similar protocols

IL2 in supernatants collected from coculture experiments were quantified using an IL2 ELISA kit (eBioscience 88-7024-22). Experiments were performed according to the manufacturers’ instructions.

Li S.X., Barrett B.S., Guo K., Kassiotis G., Hasenkrug K.J., Dittmer U., Gibbert K, & Santiago M.L. (2016). Tetherin/BST-2 promotes dendritic cell activation and function during acute retrovirus infection. Scientific Reports, 6, 20425.

+ Open protocol

+ Expand

Comprehensive Characterization of Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H nuclear magnetic resonance (¹H NMR) spectra were recorded on a Bruker AV-300 NMR spectrometer in CDCl₃, TFA-d, D₂O, or DMSO-d₆. The molecular weight was determined by gel permeation chromatography (GPC, SA). The size distribution of nanoparticles was determined by dynamic laser scattering (DLS) while their zeta potential was measured by a Zeta Potential/BI-90Plus particle size analyzer (Brookhaven Instruments Corporation, USA). The concentration of unloaded IL-2 was quantified by an IL-2 ELISA kit (Thermo Fisher, USA). Transmission electron microscope (TEM) images were obtained from the JEOL JEM-1011 (Tokyo, Japan). Fluorescence intensity was measured using a microplate reader (Bio-Rad, Hercules, CA, USA). Histological changes were observed with an optical microscope (Nikon Eclipse Ti, Optical Instruments Corporation, Ardmore, PA, USA). Lymphocytes were measured by using flow cytometry (BD FACS Celesta, USA). Using FlowJo software (Becton Dickinson and Company, USA), cell populations were identified and quantified.

Wang D., Wang X., Zhang Y., Yu L., An J., Wang X., Huang Y, & Han X. (2024). The combination of IL-2 nanoparticles and Palbociclib enhances the anti-tumor immune response for colon cancer therapy. Frontiers in Immunology, 15, 1309509.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!