RNAs were isolated according to the protocol of TRzol reagent (Beyotime) with minor modification. cDNA was synthesized using RT reagent kit with gDNA Eraser (Beyotime). SYBR Green Abstart PCR Mix (Sangon Biotechnology Inc.) was used to perform qPCR on Toptical 96 Real-Time PCR System. β-actin were used as endogenous controls. The detailed primer information is listed in Supplementary Table 2 .
Sybr green abstart pcr mix
Manufactured by Sangon
SYBR Green Abstart PCR Mix is a ready-to-use solution for real-time PCR amplification. It contains SYBR Green I, a fluorescent dye that binds to double-stranded DNA, and all the necessary components for PCR.
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Lab products found in correlation
2 protocols using sybr green abstart pcr mix
1
RNA Isolation and qPCR Analysis
2
RNA Extraction and RT-qPCR Analysis
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By means of Trizol reagent (Sangon Biotech), total RNA was acquired. 1 μg RNA was subjected to reverse transcription through RevertAid First Strand cDNA Synthesis Kit (Sangon Biotech), in compliance with the instructions. PCR amplification was conducted with SYBR Green Abstart PCR Mix (Sangon Biotech). Relative mRNA expression was calculated by the comparative Ct method with GAPDH serving as normalization 13 (link).
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