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Withaccutase

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Withaccutase is a recombinant cell dissociation enzyme designed for gentle and efficient dissociation of adherent cells from cell culture surfaces. It is suitable for use in a variety of cell culture applications.

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Lab products found in correlation

Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (4 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions) Geltrex, by Thermo Fisher Scientific (2 mentions) Normocin, by InvivoGen (2 mentions) Rho associated kinase rock inhibitor, by Merck Group (2 mentions)

2 protocols using withaccutase

1

Culturing Human Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Embryonic Stem Cell (hESC) line (HUES66) was obtained from the
Harvard Stem Cell Institute. Stem cells were cultured in 10 cm tissue culture
dishes coated with 5 ml of geltrex (Thermo-Fisher Scientific) diluted in DMEM
(Thermo-Fisher Scientific) with 1:100 ratio for 30 min at 37 °C. Stem
cell culture medium contained mTeSR (Stemcell Technologies) with provided
supplement and normocin (InvivoGen). Stem cells were passaged by detaching with
accutase (Stemcell Technologies) diluted in DPBS (Thermo-Fisher Scientific) at
1:3. After each splitting of stem cells, 10μM Rho-associated kinase
(ROCK) inhibitor (EMD Millipore) was added in medium of re-plated cells. Stem
cell culture medium was replaced daily.
Tekin H., Simmons S., Cummings B., Gao L., Adiconis X., Hession C.C., Ghoshal A., Dionne D., Choudhury S.R., Yesilyurt V., Sanjana N.E., Shi X., Lu C., Heidenreich M., Pan J.Q., Levin J.Z, & Zhang F. (2018). Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature biomedical engineering, 2(7), 540-554.
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2

Culturing Human Embryonic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human Embryonic Stem Cell (hESC) line (HUES66) was obtained from the
Harvard Stem Cell Institute. Stem cells were cultured in 10 cm tissue culture
dishes coated with 5 ml of geltrex (Thermo-Fisher Scientific) diluted in DMEM
(Thermo-Fisher Scientific) with 1:100 ratio for 30 min at 37 °C. Stem
cell culture medium contained mTeSR (Stemcell Technologies) with provided
supplement and normocin (InvivoGen). Stem cells were passaged by detaching with
accutase (Stemcell Technologies) diluted in DPBS (Thermo-Fisher Scientific) at
1:3. After each splitting of stem cells, 10μM Rho-associated kinase
(ROCK) inhibitor (EMD Millipore) was added in medium of re-plated cells. Stem
cell culture medium was replaced daily.
Tekin H., Simmons S., Cummings B., Gao L., Adiconis X., Hession C.C., Ghoshal A., Dionne D., Choudhury S.R., Yesilyurt V., Sanjana N.E., Shi X., Lu C., Heidenreich M., Pan J.Q., Levin J.Z, & Zhang F. (2018). Effects of 3D culturing conditions on the transcriptomic profile of stem-cell-derived neurons. Nature biomedical engineering, 2(7), 540-554.
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