Imipenem

Manufactured by Meilun

Sourced in China

Imipenem is a broad-spectrum antibiotic used in the treatment of various bacterial infections. It functions as a carbapenem drug, inhibiting cell wall synthesis in susceptible bacteria.

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Lab products found in correlation

9 protocols using imipenem

Microbiota Depletion in Mice

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For specific deletion of colon microbiota, mice were treated by enema with 500 µl ABX solution containing neomycin (#MB1716, Meilunbio, 2 g L⁻¹), vancomycin (#MB1260, Meilunbio, 1 g L⁻¹), and imipenem (#MB1748, Meilunbio, 0.5 g L⁻¹) twice a day for 2 weeks.
For depletion of microbiota along the whole gut, mice were treated with an ABX solution containing neomycin (#MB1716, Meilunbio, 1 g L⁻¹), vancomycin (#MB1260, Meilunbio, 0.5 g L⁻¹), and imipenem (#MB1748, Meilunbio, 0.25 g L⁻¹). ABX was dissolved into the sterile drinking water. Solutions and bottles were changed twice a week. Mice were treated for two weeks before tumor implantation and were continuously treated throughout the experiment.

Pu C., Li Y., Fu Y., Yan Y., Tao S., Tang S., Gai X., Ding Z., Gan Z., Liu Y., Cao S., Wang T., Ding J., Xu J., Geng M, & Huang M. (2024). Low‐Dose Chemotherapy Preferentially Shapes the Ileal Microbiome and Augments the Response to Immune Checkpoint Blockade by Activating AIM2 Inflammasome in Ileal Epithelial Cells. Advanced Science, 11(11), 2304781.

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Antibacterial Agents: Comprehensive Evaluation

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A wide range of clinically-used antibacterial agents including ampicillin, ampicillin-sulbactam (2:1), cefepime, ceftazidime, doxycycline, minocycline, amikacin, gentamicin, ciprofloxacin, levofloxacin, chloramphenicol, imipenem, meropenem, tigecycline, polymyxin B, azithromycin and rifampicin were purchased from Meilun Biological (Dalian, Liaoning, China). Zinc lactate, stannous fluoride and furanone were obtained, respectively, from Yuanye Bio-Technology (Shanghai, China), Macklin Biochemical (Shanghai), and Sigma-Aldrich (St. Louis, Missouri, USA). Bacterial culture media, Muller-Hinton broth (MHB), cation-adjusted MH broth (CAMHB), and tryptic soy broth (TSB) medium were purchased from Haibo Biotechnology (Qingdao, Shandong, China).

Peng Q., Lin F, & Ling B. (2020). In vitro activity of biofilm inhibitors in combination with antibacterial drugs against extensively drug-resistant Acinetobacter baumannii. Scientific Reports, 10, 18097.

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Imipenem-Cilastatin Pharmacokinetic Study

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Imipenem was supplied from Dalian Meilun Biology Technology Co., Ltd. (Dalian, China). Cilastatin was extracted from Imipenem and cilastatin sodium for injection. Probenecid, PAH, PCG, estrone-3-sulfate (ES), and tetraethyl ammonium (TEA) were purchased from Sigma (St. Louis, MO, USA). All other reagents and chemicals in this study were of analytical purity grade and were commercially available.

Zhu Y., Huo X., Wang C., Meng Q., Liu Z., Sun H., Tan A., Ma X., Peng J, & Liu K. (2018). Organic anion transporters also mediate the drug–drug interaction between imipenem and cilastatin. Asian Journal of Pharmaceutical Sciences, 15(2), 252-263.

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Screening Antibiotic Resistance Genes and Potential Inhibitors

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The bacterial strains, plasmids and primers used in this study are listed in Supplementary Table S1, Table S2 and Table S3, respectively. All bacterial strains were grown on Luria-Bertani (LB) plates or in LB broth. All NDM-1-positive strains were originated from our previous studies [17] . E. cloacae 20710 and E. cloacae 20712 were provided by Dr. Yonghong Xiao at Zhejiang University [18] . A. baumannii 21 was provided by Dr. Zhimin Guo at the First Hospital of Jilin University.
The full gene sequences of blaIMP-1, blaVIM-1, blaKPC-2 and blaOXA-48 were synthesized by GeneScript (Nanjing, China) and were inserted into pET28a or pETSUMO for protein expression.
The FDA-approved drug library was purchased from ApexBio Technology (Cat#L1021, Houston, TX, USA). Dexrazoxane, embelin, CAN and NDGA were dissolved in DMSO (Sigma-Aldrich, St. Louis, MO, USA). Meropenem, amoxicillin, ciprofloxacin, imipenem, erythromycin, chloramphenicol, tetracycline and gentamicin were obtained from Dalian Meilun Biotechnology Co., Ltd. (Dalian, China).

Guo Y., Liu H., Yang M., Ding R., Gao Y., Wang H., Deng X., Wang J., Feng H., & Qiu J. (2022). Novel MBLs inhibitors screened from FDA-approved drug library restore the susceptibility of carbapenems to NDM-1-harbouring bacteria.

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Antibacterial Agents Procurement and Media Preparation

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Clinically-used antibacterial agents (Magiorakos et al., 2012 (link)), such as piperacillin, ceftazidime, cefotaxime, tetracycline, minocycline, gentamicin, amikacin, ciprofloxacin, levofloxacin, chloramphenicol, imipenem, meropenem, tigecycline, polymyxin B, polymyxin E, vancomycin, bacitracin, and teicoplanin were purchased from Meilun Biological (Dalian, Liaoning, China). In addition, cation-adjusted MH broth (CAMHB), Muller-Hinton broth (MHB) and tryptic soy broth (TSB) medium were obtained from Haibo Biotechnology (Qingdao, Shandong, China).

Meng Q., Lin F, & Ling B. (2022). In Vitro Activity of Peptide Antibiotics in Combination With Other Antimicrobials on Extensively Drug-Resistant Acinetobacter baumannii in the Planktonic and Biofilm Cell. Frontiers in Pharmacology, 13, 890955.

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Antibiotics and Biocides Evaluation

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The antibiotics and biocides used in this study were purchased commercially. Chlorhexidine acetate, Doxycycline (DOX), minocycline (MIN), levo oxacin (LEV), cipro oxacin (CIP), imipenem (IMP), meropenem (MER) were purchased from Dalian Meilun Biological Technology (Dalian, China).

Lin F., Yu B., Wang Q., Yuan M., & Ling B. (2021). Combination Inhibition Activity of Chlorhexidine and Antibiotics on Multidrug-Resistant Acinetobacter Baumannii in Vitro.

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Antimicrobial Susceptibility Testing Protocol

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Susceptibility to antimicrobials was determined in triplicate using the standard agar dilution method recommended by the Clinical and Laboratory Standards Institute (CLSI, 2020) [66 ]. The following eighteen antimicrobials were tested: kanamycin (KAN), gentamicin (GEN), erythromycin (ERY), azithromycin (AZM), norfloxacin (NOR), ofloxacin (OFX), ciprofloxacin (CIP), lomefloxacin (LOM), levofloxacin (LEV), sulfadiazine (SD), trimethoprim (TMP), ceftriaxone (CRO), cefixime (CFM), ampicillin (AMP), amoxicillin (AML), aztreonam (ATM), imipenem (IPM) and tetracycline (TET) (Meilun Biotechnology Co., LTD, Dalian, China). The isolates were grown on TSA plates, suspended in stroke-physiological saline solution to a turbidity equivalent to 0.5 McFarland Standard, and inoculated onto Mueller-Hinton agar plates using a multipoint inoculator (MIT-60P; Sakuma Seisakusyo, Tokyo, Japan). The final inoculum was approximately 10⁴ CFU per spot. Plates were incubated at 37 °C for 16–18 h. The range of 2-fold concentrations used to determine the susceptibility was determined by CLSI criteria. In addition, the results were interpreted in accordance with CLSI criteria (Supplementary Table S3). E. coli ATCC25922 and E. faecalis ATCC29212 were used as quality control strains.

Wang X., Zhang Y., Li C., Li G., Wu D., Li T., Qu Y., Deng W., He Y., Penttinen P., Zhang H., Huang Y., Zhao K, & Zou L. (2022). Antimicrobial resistance of Escherichia coli, Enterobacter spp., Klebsiella pneumoniae and Enterococcus spp. isolated from the feces of giant panda. BMC Microbiology, 22, 102.

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Antimicrobial Agents Evaluation

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Two carbapenem drugs, namely imipenem (catalog no. MB1457) and meropenem (catalog no. MB1129), were purchased from Meilunbio (Dalian, China). The glycylcycline drug tigecycline (catalog no. E129449) was purchased from Aladdin (Shanghai, China). The novel fluorocycline drug eravacycline (catalog no. A13887-10) was purchased from AdooQ BioScience (Irvine, CA, USA).

Zheng J.X., Lin Z.W., Sun X., Lin W.H., Chen Z., Wu Y., Qi G.B., Deng Q.W., Qu D, & Yu Z.J. (2018). Overexpression of OqxAB and MacAB efflux pumps contributes to eravacycline resistance and heteroresistance in clinical isolates of Klebsiella pneumoniae. Emerging Microbes & Infections, 7, 139.

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Commercially Available Antimicrobial Agents

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The antibiotics and biocides used in this study were purchased commercially. Chlorhexidine acetate, Doxycycline (DOX), minocycline (MIN), levofloxacin (LEV), ciprofloxacin (CIP), imipenem (IMP), meropenem (MER) were purchased from Dalian Meilun Biological Technology (Dalian, China).

Lin F., Yu B., Wang Q., Yuan M, & Ling B. (2021). Combination inhibition activity of chlorhexidine and antibiotics on multidrug-resistant Acinetobacter baumannii in vitro. BMC Infectious Diseases, 21, 266.

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