A total of 5 Sprague-Dawley rats (WT) and 11 rats expressing human IAPP (HIP rats) at ~7 months of age were used for current study. Rats were bred and housed in pairs under standard environmental conditions with a 12-hr light-dark (LD) cycle and habituated to routine handling, restraint, and oral gavage. After 12 h food deprivation, rats were administered an oral glucose tolerance test (OGTT) by oral gavage of 1.5 g/kg body weight of dextrose and blood was collected via lateral saphenous venipuncture into chilled, EDTA-treated microcentrifuge tubes before (0 min) and 10, 20, 40, 60, 90, and 120 min post oral gavage. Plasma glucose was measured by the glucose oxidase method using the YSI 2300 STAT Plus Glucose & Lactate Analyzer (YSI Life Sciences). Plasma insulin and C-peptide were measured using the ALPCO Diagnostics Rat Insulin ELISA kit (80-INSRT-E01) and Rat C-peptide ELISA kit (80-CPTRT-E01), respectively. Plasma glucagon was measured using the Mercodia Glucagon ELISA kit (10-1271-01).
Rat c peptide elisa kit
Manufactured by ALPCO
Sourced in United States
The Rat C-Peptide ELISA Kit is a laboratory assay used to quantitatively measure the concentration of C-peptide in rat samples. C-peptide is a byproduct of insulin production and its measurement can provide information about insulin secretion.
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2 protocols using rat c peptide elisa kit
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Oral Glucose Tolerance Test in Rat Model
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Rat C-peptide Measurement by ELISA
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Rat blood was collected from the femoral vein under anesthesia into BD Vacutainer® Rapid Serum Tubes, left for 30 min at room temperature, then centrifuged and serum was collected. The concentration of C‐peptide was measured with rat C‐peptide ELISA kit (ALPCO Diagnostics, Salem, NH, USA).
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