Ortho phosphoric acid, by Scharlab - Product details

1

Synthesis of Endocrine-Disrupting Compounds

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The reagents used in this work are described in the following list:

Androsterone 97,7%, Sigma Aldrich, CAS Number: 53–41-8.

Bisphenol A 99,5%, Dr Ehrenstorfer, CAS Number: 80–05-7.

Estrone 99,3%, Sigma Aldrich, CAS Number: 53–16-7.

Graphite powder, BAYCARBON Inc., Product SP-1.

Hydrochloric Acid, Sigma Aldrich, CAS Number: 7647–01-0.

Hydrogen Peroxide, Chem-Lab, CAS Number: 7722–84-1.

Nitric Acid, Sigma Aldrich, CAS Number: 7697–37-2.

19-Norethindrone 99.6%, Sigma Aldrich, CAS Number: 68–22-4.

Orthophosphoric Acid, Scharlau, CAS Number: 7664–38-2.

Potassium Bromide, Fluka, CAS Number: 7758–02-3.

Potassium Permanganate, Merck, CAS Number: 7722–64-7.

Sulfuric Acid, Sigma Aldrich, CAS Number: 7664–93-9.

Vanadium Pentoxide, Merck, CAS Number: 1314–62-1.

17α-Ethinylestradiol 99,5%, Dr Ehrenstorfer, CAS Number: 57–63-6.

2,4-Dichlorophenol 99,7%, Supelco, CAS Number: 120–83-2.

Regkouzas P., Sygellou L, & Diamadopoulos E. (2023). Production and characterization of graphene oxide-engineered biochars and application for organic micro-pollutant adsorption from aqueous solutions. Environmental Science and Pollution Research International, 30(37), 87810-87829.

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2

HPLC Analysis of Extracted Compounds

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All solvents used were HPLC grade, and all reagents were analytical grade. The analysis was performed using a high-performance liquid chromatography system (Hitachi D2000), which consisted of an L-2130 HTA Pump, an L-2200 autosampler, and an L-2455 diode array detector. The extracted compound was separated using an Inertsil ODS-2 C18 column (250 mm × 4.6 mm, 5 μm) with a gradient mobile phase of 0.3% ortho-phosphoric acid (Scharlab) in ddH₂O (solvent A) and acetonitrile (Fisher Chemical) (solvent B). HPLC was performed at a flow rate of 0.8 ml/min with detection at 220 nm. The column temperature was maintained at 30°C. All samples were diluted in methanol (Honeywell) before analysis. Chromatographic peaks were identified on the basis of retention time.

Lo C.W., Pi C.C., Chen Y.T, & Chen H.W. (2020). Vigna radiata (L.) R. Wilczek Extract Inhibits Influenza A Virus by Targeting Viral Attachment, Penetration, Assembly, and Release. Frontiers in Pharmacology, 11, 584973.

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3

Screening Antioxidant Capacity of Onion Extracts

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For the film preparation, medium-sized yellow onion bulbs in commercial stages were acquired from markets in the city of São Carlos, São Paulo, Brazil. For the incorporation of clay, Laponite XLG (Na_0.7[(Mg_5.5Li_0.3)Si₈O₂₀(OH)₄]) obtained from BYK-Chemie was used for clay incorporation. Milli-Q^® water, sodium salicylate (CAS 54-21-7, 99.5%), 2,5-dihydroxybenzoic acid (CAS 490-79-9, 99%) from Sigma-Aldrich, HPLC-grade methanol (CAS 67-56-1), hydrogen peroxide (CAS 7722-84-1, 30%) and orthophosphoric acid (7664-38-2, 84%) from Scharlab were used for the antioxidant assay.

Barbosa M.L., de Oliveira L.M., Paiva R., Dametto A.C., Dias D.D., Ribeiro C.A., Wrona M., Nerín C., Barud H.D, & Cruz S.A. (2023). Evaluation the Potential of Onion/Laponite Composites Films for Sustainable Food Packaging with Enhanced UV Protection and Antioxidant Capacity. Molecules, 28(19), 6829.

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4

Quantifying NSAID Metabolite Profiles

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The NSAIDs diclofenac (sodium salt, D6899, DIC), ketoprofen (≥ 98% TLC, K1751, KET), naproxen (≥ 98%, sodium salt, M1275, NAP) and Ibuprofen (sodium salt, ≥ 98% GC, I1892, IBU) were purchased from Sigma-Aldrich. Stocks solutions of 4 mg ml -1 for each NSAIDs were prepared in Methanol (LC-MS grade, Merck). For the confirmation of the metabolites, Prostaglandin E1 (P5515) and Arachidonic acid (23401) standards from Sigma Aldrich were used. Lipid extraction was based on the method of Zöllner and Kirsch (1962) , using Chloroform (HPLC grade, Merck), Methanol (HPLC grade, Sigma Aldrich) for the extraction and for the analysis Vanillin (99%, V1104, Sigma Aldrich) and orthophosphoric acid (85%, reagent grade, Scharlau) were used. The mobile phase used for metabolomics approach was HPLC grade acetonitrile and water purchased from Merck (Darmstadt, Germany).

López-Doval J.C., Serra-Compte A., Rodríguez-Mozaz S., Barceló D, & Sabater S. (2020). Diet quality and NSAIDs promote changes in formation of prostaglandins by an aquatic invertebrate. Chemosphere, 257.

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5

Synthesis of alpha-cyclodextrin conjugates

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Alpha-cyclodextrin, N,N′-carbonyldi imidazole (CDI), imidazole, and ammonium dihydrogen phosphate were purchased from Sigma Aldrich (St. Louis, MO, USA). Milli Q water was generated using an Ultramatic Wasserlab purification system (Pamplona, Spain). For pH adjustment, 37% hydrochloric acid and 85% ortho-phosphoric acid (Scharlab, Madrid, Spain) were used. Analytical grade acetonitrile was purchased from Honeywell (Charlotte, NC, USA). Bentonites and zeolites were kindly provided by Nurel S.A. (Zaragoza, Spain).

Rupérez D., Gracia-Vallés N., Clavero E., Silva F, & Nerín C. (2022). Mechanochemically Scaled-Up Alpha Cyclodextrin Nanosponges: Their Safety and Effectiveness as Ethylene Scavenger. Nanomaterials, 12(17), 2900.

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6

Moroccan Pine Cone-Based Activated Carbon for Bisphenol A Removal

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Moroccan pine cones (Pinus halepensis) were collected locally from a forest in the province of Sefrou, in the Fez-Meknes region, Morocco. Zinc chloride (ZnCl₂), and ortho-phosphoric acid, (H₃PO₄, 85 % w/w.) were purchased from Scharlau (Spain). Bisphenol A (≥99 % purity) was procured from Sigma Aldrich (Germany). Hydrochloric acid (HCl, ≥37 %), and sodium hydroxide (NaOH, ≥97 %) were purchased from Fluka (Germany). Ethanol was supplied by Biosmart (Morocco). Reverse osmosis (RO) water was prepared in the laboratory using an RO purification system (Elettronica Veneta) and used to prepare BPA solutions for adsorption experiments. All reagents were used as received without further purification. Table 1 presents the representative emerging contaminant selected for assessing the performance of the prepared activated carbons for water treatment: Bisphenol A (BPA).Table 1

General physicochemical properties of BPA [19 (link)].

Table 1

Properties	Bisphenol A
Molecular Formula	C₁₅H₁₆O₂
Chemical structure	Image 1
Density (g.cm⁻³)	1.20
Molecular weight (g.mol⁻¹)	228.291
Water solubility (mg. L⁻¹)	120–300
Dissociation constant, pK_a	10.29

Jari Y., Roche N., Chaker Necibi M., Zahra Falil F., Tayibi S., Lyamlouli K., Chehbouni A, & Gourich B. (2024). Porous activated carbons derived from waste Moroccan pine cones for high-performance adsorption of bisphenol A from water. Heliyon, 10(9), e29645.

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7

Screening Antioxidant Capacity of Onion Extracts

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For the film preparation, medium-sized yellow onion bulbs in commercial stages were acquired from markets in the city of São Carlos, São Paulo, Brazil. For the incorporation of clay, Laponite XLG (Na_0.7[(Mg_5.5Li_0.3)Si₈O₂₀(OH)₄]) obtained from BYK-Chemie was used for clay incorporation. Milli-Q^® water, sodium salicylate (CAS 54-21-7, 99.5%), 2,5-dihydroxybenzoic acid (CAS 490-79-9, 99%) from Sigma-Aldrich, HPLC-grade methanol (CAS 67-56-1), hydrogen peroxide (CAS 7722-84-1, 30%) and orthophosphoric acid (7664-38-2, 84%) from Scharlab were used for the antioxidant assay.

Barbosa M.L., de Oliveira L.M., Paiva R., Dametto A.C., Dias D.D., Ribeiro C.A., Wrona M., Nerín C., Barud H.D, & Cruz S.A. (2023). Evaluation the Potential of Onion/Laponite Composites Films for Sustainable Food Packaging with Enhanced UV Protection and Antioxidant Capacity. Molecules, 28(19), 6829.

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8

Favipiravir Analytical Method Development

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Favipiravir was kindly supplied by Optrix Laboratories private Limited, India. Lamivudine, used as an internal standard (IS), was bought from LGC GmbH, Germany. Potassium dihydrogen phosphate, sodium dihydrogen phosphate, ortho phosphoric acid, acetic acid, ammonium formate and formic acid were purchased from Scharlau, Spain. Methanol and acetonitrile (HPLC grade), Sigma Aldrich, Germany.

Bendas E.R., Rezk M.R, & Badr K.A. (2023). Does the Ethnic Difference Affect the Pharmacokinetics of Favipiravir? A Pharmacokinetic Study in Healthy Egyptian Volunteers and Development of Level C In-vitro In-vivo Correlation. Drug research, 73(6).

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9

Phytochemical Analysis of Licorice Extracts

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CO 2 (99.98 % purity) was supplied from Carburos Metálicos (Madrid, Spain). Ethanol (99.8 % purity), Sodium Carbonate anhydrous (99.5% purity) and Folin-Ciocalteu´s reagent were purchased from Panreac (Barcelona, Spain). Gallic acid standard (> 98% purity), 2,2-Diphenyl-1-pycrilhydrazyl (DPPH, 95% purity), (±)-6-Hydroxy-2,5,7,8tetramethyllchromane-2-carboxylic acid (Trolox, 97% purity), liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Orthophosphoric acid (85% purity) was purchased from Scharlab S.L. (Sentmenat, Spain). Acetonitrile (99,8% purity) was purchased from Macron (Poland).

Quintana S.E., Hernández D.M., Villanueva-Bermejo D., García‐Risco M.R., & Fornari T. (2020). Fractionation and precipitation of licorice (Glycyrrhiza glabra L.) phytochemicals by supercritical antisolvent (SAS) technique. LWT, 126, 109315-109315.

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10

Phytochemical Analysis of Licorice Extracts

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CO 2 (99.98 % purity) was supplied from Carburos Metálicos (Madrid, Spain). Ethanol (99.8 % purity), Sodium Carbonate anhydrous (99.5% purity) and Folin-Ciocalteu´s reagent were purchased from Panreac (Barcelona, Spain). Gallic acid standard (> 98% purity), 2,2-Diphenyl-1-pycrilhydrazyl (DPPH, 95% purity), (±)-6-Hydroxy-2,5,7,8tetramethyllchromane-2-carboxylic acid (Trolox, 97% purity), liquiritin, liquiritigenin, isoliquiritigenin, glabridin and glycyrrhizic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Orthophosphoric acid (85% purity) was purchased from Scharlab S.L. (Sentmenat, Spain). Acetonitrile (99,8% purity) was purchased from Macron (Poland).

Quintana S.E., Hernández D.M., Villanueva-Bermejo D., García-Risco M.R, & Fornari T. (2020). Fractionation and precipitation of licorice (Glycyrrhiza glabra L.) phytochemicals by supercritical antisolvent (SAS) technique. Lebensmittel-Wissenschaft + [i.e. und] Technologie. Food science + technology. Science + technologie alimentaire, 126.

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Ortho phosphoric acid

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10 protocols using ortho phosphoric acid

Synthesis of Endocrine-Disrupting Compounds

HPLC Analysis of Extracted Compounds

Screening Antioxidant Capacity of Onion Extracts

Quantifying NSAID Metabolite Profiles

Synthesis of alpha-cyclodextrin conjugates

Moroccan Pine Cone-Based Activated Carbon for Bisphenol A Removal

Screening Antioxidant Capacity of Onion Extracts

Favipiravir Analytical Method Development

Phytochemical Analysis of Licorice Extracts

Phytochemical Analysis of Licorice Extracts

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