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Sb206553

Manufactured by Bio-Techne
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SB206553 is a small molecule compound. It is a selective antagonist of the prostaglandin E2 receptor EP4.

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Lab products found in correlation

Vasopressin, by Bio-Techne (2 mentions) L phenyalanine, by Merck Group (2 mentions) Gr127935, by Bio-Techne (2 mentions) Nsd1015, by Merck Group (2 mentions) Rx821002, by Bio-Techne (2 mentions) Sb242084, by Bio-Techne (1 mentions) Ondansetron, by Bio-Techne (1 mentions) Way161503, by Bio-Techne (1 mentions)

3 protocols using sb206553

1

Detailed ACSF compositions for in vitro experiments

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Two kinds of ACSF were used in in vitro experiments: mACSF in the dissection dish before recording and nACSF in the recording chamber. mACSF was composed of (in mM) 118 NaCl, 24 NaHCO3, 1.5 CaCl2, 3 KCl, 5 MgCl2, 1.4 NaH2PO4, 1.3 MgSO4, 25 d-glucose, and 1 kynurenic acid. nACSF was composed of (in mM) 122 NaCl, 25 NaHCO3, 2.5 CaCl2, 3 KCl, 1 MgCl2, 0.5 NaH2PO4, and 12 d-glucose. Both types of ACSF were saturated with carbogen (95% O2-5% CO2) and maintained at pH 7.4. The drugs added to the nACSF were 5-HTP, clorgyline, pargyline, tryptamine, L-tryptophan, tyramine, L-tyrosine, 2-phenylethylamine, L-phenyalanine, NSD1015 (Sigma-Aldrich), α-methyl-5-HT, octopamine, SB206553, RX821002, GR127935, dobutamine, vasopressin (Tocris) and zolmitriptan (AstraZenica). All drugs were first dissolved as a 10 – 50 mM stock solution in water before final dilution in ACSF for in vitro ventral root reflexes recording and DIC microscopy or in saline for in vivo oxygen measurements and two photon microscopy, with the exception of zolmitriptan, which was dissolved in minimal amounts of DMSO (DMSO final concentration in nACSF was 0.04%). DMSO alone had no effect on in vitro LLR in vehicle controls, as compare to nACSF control state.
Li Y., Lucas-Osma A.M., Black S., Bandet M.V., Stephens M.J., Vavrek R., Sanelli L., Fenrich K.K., Di Narzo A.F., Dracheva S., Winship I.R., Fouad K, & Bennett D.J. (2017). Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nature medicine, 23(6), 733-741.
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2

Pharmacological Modulation of 5-HT Receptors

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Fluoxetine (FLX; ANAWA Trading SA, Wangen, Switzerland), was dissolved in 0.9% NaCl or drinking water to achieve 2.5, 5, or 10 mg/kg when administered intraperitoneally (i.p.) or through the drinking water (see supplementary data). The 5-HT2C receptor inverse agonist SB206553 (Tocris, Ellisville, MO), the 5-HT2C receptor antagonist SB242084 (Tocris), the 5-HT2C receptor agonist WAY161503 (Tocris), and the 5-HT3 receptor antagonist ondansetron (Tocris) were dissolved by sonication in 0.9% NaCl to achieve concentrations between 0.1 and 5 mg/kg when administered i.p.. For local infusions, SB242084 was first dissolved in DMSO to 10 mg/ml, and then diluted at 1:100 in saline to obtain a final concentration of 0.1 mg/ml.
Demireva E.Y., Suri D., Morelli E., Mahadevia D., Chuhma N., Teixeira C.M., Ziolkowski A., Hersh M., Fifer J., Bagchi S., Chemiakine A., Moore H., Gingrich J.A., Balsam P., Rayport S, & Ansorge M.S. (2018). 5-HT2C receptor blockade reverses SSRI-associated basal ganglia dysfunction and potentiates therapeutic efficacy. Molecular Psychiatry, 25(12), 3304-3321.
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3

Detailed ACSF compositions for in vitro experiments

Check if the same lab product or an alternative is used in the 5 most similar protocols
Two kinds of ACSF were used in in vitro experiments: mACSF in the dissection dish before recording and nACSF in the recording chamber. mACSF was composed of (in mM) 118 NaCl, 24 NaHCO3, 1.5 CaCl2, 3 KCl, 5 MgCl2, 1.4 NaH2PO4, 1.3 MgSO4, 25 d-glucose, and 1 kynurenic acid. nACSF was composed of (in mM) 122 NaCl, 25 NaHCO3, 2.5 CaCl2, 3 KCl, 1 MgCl2, 0.5 NaH2PO4, and 12 d-glucose. Both types of ACSF were saturated with carbogen (95% O2-5% CO2) and maintained at pH 7.4. The drugs added to the nACSF were 5-HTP, clorgyline, pargyline, tryptamine, L-tryptophan, tyramine, L-tyrosine, 2-phenylethylamine, L-phenyalanine, NSD1015 (Sigma-Aldrich), α-methyl-5-HT, octopamine, SB206553, RX821002, GR127935, dobutamine, vasopressin (Tocris) and zolmitriptan (AstraZenica). All drugs were first dissolved as a 10 – 50 mM stock solution in water before final dilution in ACSF for in vitro ventral root reflexes recording and DIC microscopy or in saline for in vivo oxygen measurements and two photon microscopy, with the exception of zolmitriptan, which was dissolved in minimal amounts of DMSO (DMSO final concentration in nACSF was 0.04%). DMSO alone had no effect on in vitro LLR in vehicle controls, as compare to nACSF control state.
Li Y., Lucas-Osma A.M., Black S., Bandet M.V., Stephens M.J., Vavrek R., Sanelli L., Fenrich K.K., Di Narzo A.F., Dracheva S., Winship I.R., Fouad K, & Bennett D.J. (2017). Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nature medicine, 23(6), 733-741.
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