All analytical grade reagents and chemicals used in this work were purchased from Sigma-Aldrich and were similar to those previously described [44 ]. Superoxide dismutase (SOD) was purchased from Bio-Diagnostic Co. (Egypt). Annexin V- FITC apoptosis detection kit was purchased from Immunostep Co. (Spain). RT-qPCR reagents, including primers (25 nM), desalt purification, RNA extraction, cDNA synthesis kits, and Evergreen Master mix were obtained from ThermoFisher Scientific (USA).
Superoxide dismutase sod
Manufactured by Biodiagnostic
Sourced in Egypt
Superoxide dismutase (SOD) is an enzyme that catalyzes the conversion of superoxide radicals into oxygen and hydrogen peroxide. It is an important antioxidant that helps protect cells from oxidative stress.
Automatically generated - may contain errors
Lab products found in correlation
Rt qpcr reagents, by Thermo Fisher Scientific (1 mentions)
Annexin 5 fitc apoptosis detection kit, by Immunostep (1 mentions)
Cdna synthesis kit, by Thermo Fisher Scientific (1 mentions)
Creatinine, by Biodiagnostic (1 mentions)
Total protein, by Biodiagnostic (1 mentions)
Albumin, by Biodiagnostic (1 mentions)
Malondialdehyde mda, by Biodiagnostic (1 mentions)
Glutathione peroxidase gpx, by Biodiagnostic (1 mentions)
Kinetin kn, by Merck Group (1 mentions)
Histopaque 1077, by Merck Group (1 mentions)
5 protocols using superoxide dismutase sod
1
Biochemical and Apoptotic Assays
2
Biosafety Evaluation of Silver Nanoparticles
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Silver nanoparticles (AgNPs) with average size (15 ± 3 nm) were purchased from Nanotech Egypt for Photo-Electronics (El-Wahaat Road, Dream Land City, Entrance 3, City of 6 October, Giza, Egypt. Vials were diluted by phosphate buffer saline (PBS) and the concentration was adjusted to 0.25 mg/kg, in 200 µl (Hemen Moradi-Sardareha et al., 2018) . Aspartate amino transferase (AST), alanine aminotransferase (ALT), urea, creatinine, albumin, total protein, superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH) and malondialdehyde (MDA) kits were purchased from Bio diagnostic company, Egypt.
3
Neuroprotective Effects of Pharmacological Compounds
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CPF was taken up from Egyptian Pesticides and Chemicals Company (EPIC), Cairo, Egypt. TQ and LP were supplied from Sigma Aldrich (Saint Louis, MO, USA), purity ≥ 98% and 90%, respectively. AchE was obtained from R&D (Mannheim, Germany). Interleukin-1β (IL-1β) and interleukin-6 (IL-6) were gained from Glory Science Co. Ltd. (Del Rio, TX, USA) and tumor necrosis factor-α (TNF-α) was bought from BioSource International Inc. (Camarillo, CA, USA). Diagnostic kits for assessment of malondialdehyde (MDA), reduced glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) were gained from Biodiagnostic Company (Cairo, Egypt).
4
Biochemical and Oxidative Parameters Assay
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Alanine amino Transaminase (ALT), Aspartate amino Transaminase (AST), Alkaline Phosphatase, (ALP), Total Protein (TP) Urea, Creatinine, Triglyceride (TriG), Cholesterol (Cho.) kits from Spectrum Co. (Spain). Malondialdehyde (MDA), Catalase (CAT), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) kits from Bio-diagnostics Co. (Egypt).
5
Kinetin and Cisplatin Modulate Oxidative Stress
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Kinetin (Kn) was obtained from Sigma-Aldrich (Dorset, Germany). Cisplatin was purchased from Mylan SAS pharmaceutical company (Saint-Priest, France). Kits for reduced glutathione (GSH) (CAT No. GR2511), Glutathione peroxidase (GPx) (CAT No. GP 2524), Malondialdehyde (MDA) (CAT No. MD2529) and Superoxide dismutase (SOD) were purchased from Biodiagnostic (Cairo, Egypt). Tumor Necrosis Factor α (TNF- α), AKT and Caspase-3 polyclonal antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Rat ELISA Kits of TNF- α, CRP and Interleukin 6 (IL-6) were obtained from Elabscience biotechnology (Houston, TX, USA) while rat FAS/CD95 (Factor-Related Apoptosis) ELISA Kit was purchased from Assay Genie, Ireland (CAT No.: RTFI00768). Histopaque -1077 was purchased from Sigma-Aldrich (Dorset, Germany (CAT No. 10771). Rat ELISA Kit for pAKT/AKT was purchased from Bioassay Technology Laboratory (Shanghai, China).
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