Ras‐GTP levels were detected as previously described (Sharma et al., 2013 ). Briefly, HEK293T cells were lysed in nonionic lysis buffer (20 mM Tris‐Cl PH7.6, 137 mM NaCl, 1 mM EGTA, 1% Triton‐X‐100, 10% glycerol, 1.5 mM MgCl2) supplemented with protease inhibitor cocktails, and the Ras activity was determined using a Ras activation assay kit (Millipore). Briefly, GTP‐bound Ras levels were determined by incubating cell lysates with Raf‐1 Ras‐binding domain conjugated to agarose beads followed by an immunoblot using an anti‐pan‐Ras antibody (Millipore) and the total Ras was also detected.
Anti pan ras antibody
Manufactured by Merck Group
The Anti-pan-Ras antibody is a laboratory reagent that can detect and identify Ras proteins, which are a family of small GTPase proteins involved in various cellular processes. The antibody recognizes a conserved epitope shared among the different Ras isoforms, allowing for the detection of multiple Ras proteins within a sample.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti pan ras antibody
1
Ras-GTP Activation Assay in HEK293T Cells
2
Quantifying Active Ras Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ras-GTP levels were detected as previously described (Sharma et al., 2013 (link)). Briefly, SHSY5Y cells were lysed in non-ionic lysis buffer (20 mM Tris-Cl PH7.6, 137 mM NaCl, 1 mM EGTA, 1% Triton-X-100, 10% glycerol, 1.5 mM MgCl2) supplemented with protease inhibitor cocktails, and the Ras activity was determined using a Ras activation assay kit (Millipore). Briefly, GTP-bound Ras levels were determined by incubating cell lysates with Raf-1 Ras-binding domain conjugated to agarose beads followed by an immunoblot using an anti-pan-Ras antibody (Millipore) and the total Ras was also detected.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!