Kits for the quantification of

The experiments were approved by the Central Hospital Affiliated to Shandong First Medical University, under the number: 2020-0618-01. All experiments were performed as per institutional ethical guidelines in the Research Center of the Central Hospital Affiliated to Shandong First Medical University, Jinan, China.
In this investigation, Wistar albino rats of weight 210-240g were used. The doses of CCK-8¹⁴, Proglumide¹⁵, heaxmethonium¹⁶ were selected as per literature reports. The kits for the quantification of lactate dehydrogenase 1 (LDH-1), MB isoform of creatine kinase (CK-MB), cardiac troponins (cTnT), Bcl-2, caspase 3 and CCK were procured from MyBioSource, Inc. San Diego, CA USA.

Male Wistar albino rats (200–250 g) were employed for this study and these animals were exposed to 12 h of light and 12 h of dark in animal house. This research was approved by Animal Ethical Committee of Shenzhen Qianhai Shekou Free Trade Zone Hospital, with ethical animal approval number 20210818. The kits for the quantification of lactate dehydrogenase 1 (LDH-1), cardiac troponin (cTnT), TNF-a, melatonin were procured from MyBioSource, Inc. San Diego, CA USA. The doses of ramelteon12 (link), luzindole13 (link)
^,14 (link), hexamethonium15 (link) and 5-hydroxydecanoic acid (5-HD)16 (link) were selected based on previous studies.