Confocal images of guts were acquired on a Zeiss LSM 880 confocal microscope, using either a 20 × Plan-APOCHROMAT/0.8 numerical aperture (NA) or a 40 × oil Plan APOCHROMAT/1.4 NA objective. Confocal images are presented as maximum projections. Settings on the microscope were first adjusted on a control gut and maintained for the acquisition of the different conditions in each experiment. Images were analyzed in Fiji (Schindelin et al., 2012 (link)) or CellProfiler (Jones et al., 2008 ) and then linearly thresholded and assembled into figure panels in Photoshop Version 21.1.1 (Adobe Systems, San Jose, CA). All adjustments to contrast and other aspects of the images were performed similarly for all conditions in each experiment.
Plan apochromat 0.8 numerical aperture
Manufactured by Zeiss
The Plan-APOCHROMAT/0.8 numerical aperture is a high-performance objective lens designed for microscopy applications. It features a numerical aperture of 0.8, which allows for efficient light collection and high-resolution imaging. The lens is designed to provide a flat, distortion-free field of view, making it suitable for a wide range of microscopy techniques.
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Lab products found in correlation
2 protocols using plan apochromat 0.8 numerical aperture
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Confocal Imaging of Gut Samples
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Confocal Imaging of Gut Samples
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Confocal images of guts were acquired on a Zeiss LSM 880 confocal microscope, using either a 20× Plan-APOCHROMAT/0.8 numerical aperture (NA) or a 40× oil Plan APOCHROMAT/1.4 NA objective. Confocal images represent maximum projections.
Settings on the microscope were first adjusted on a control gut and maintained for the acquisition of the different conditions in each experiment. Images were analyzed in Fiji [76] (link) or CellProfiler [77] (link), then linearly thresholded and assembled into figure panels in Photoshop Version 21.1.1 (Adobe Systems, Inc., San Jose, CA, USA). All adjustments to contrast and other aspects of the images were performed similarly for all conditions in each experiment.
Settings on the microscope were first adjusted on a control gut and maintained for the acquisition of the different conditions in each experiment. Images were analyzed in Fiji [76] (link) or CellProfiler [77] (link), then linearly thresholded and assembled into figure panels in Photoshop Version 21.1.1 (Adobe Systems, Inc., San Jose, CA, USA). All adjustments to contrast and other aspects of the images were performed similarly for all conditions in each experiment.
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