The largest database of trusted experimental protocols

Fitc conjugated hla dr

Manufactured by BD
Sourced in United States

FITC-conjugated HLA-DR is a fluorescently labeled antibody that binds to the HLA-DR protein, which is a component of the major histocompatibility complex (MHC) class II. This product can be used for the identification and analysis of HLA-DR-expressing cells in flow cytometry applications.

Automatically generated - may contain errors

3 protocols using fitc conjugated hla dr

1

Immunophenotyping of Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunophnotypes were evaluated using mouse anti-human monoclonal antibodies: fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA) and Phycoerythin (PE)-conjugated CD73, CD90, CD105, CD11b, CD34, CD45 and CD79a. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 106 cells was incubated with monoclonal antibodies for 15 minutes at room temperature in the dark and fixed with BD CytofixTM (BD Biosciences). The analysis was performed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuestTM Proversion 6.0 software (BD Biosciences).
+ Open protocol
+ Expand
2

Immunophenotyping of Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells harvested from 3rd passage (P3) were used for immunophenotyping analysis. Mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD73, CD90, CD105, CD45, CD11b, CD34, CD79a, and FITC-conjugated HLA-DR (BD Biosciences, San Jose, CA) were used. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension, containing 1 × 106 cells, were incubated with monoclonal antibodies at room temperature in the dark and fixed with BD CytofixTM (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuestTM Proversion 6.0 software (BD Biosciences).
+ Open protocol
+ Expand
3

Macrophage Polarization Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phorbol 12-myristate 13-acetate (PMA), Lipopolysaccharides (LPS) and Hoechst 33258 dye were from Sigma-Aldrich (St. Louis, MO, USA). The cytokines for interferon γ (IFNγ), interleukin-4 (IL-4) and interleukin-13 (IL-13) were from R&D Systems (Minneapolis, MN, USA). The antibody for CD163 was from Abcam (Cambridge, MA, USA). The primary antibodies for E-cadherin, GAPDH were from Cell Signaling Technology (Danvers, MA, USA). Antibodies for fluorescein isothiocyanate (FITC)-conjugated CD68, FITC-conjugated CD206, FITC-conjugated CD163, FITC-conjugated HLA-DR and isotype control were from BD Biosciences (San Jose, CA, USA). FITC-conjugated E-cadherin antibody was from BioLegend (San Diego, CA, USA). Hepatocyte maintenance medium (HMM) and SingleQuots were from Lonza (Anaheim, CA, USA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!