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Anti fluorescence labeled anti mouse irdye680lt

Manufactured by LI COR
Sourced in Germany

The Anti-fluorescence-labeled anti-mouse IRDye680LT is a fluorescent dye-labeled antibody used for detection and quantification of mouse proteins in Western blotting and other immunoassay applications. The dye is designed to minimize background fluorescence.

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2 protocols using anti fluorescence labeled anti mouse irdye680lt

1

Antibody Characterization for MLH1 Analysis

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Anti-MLH1 (G168-728) and anti-PMS2 (A16-4) were purchased from Pharmingen (BD Biosciences, Heidelberg, Germany), anti-beta Actin (Clone AC-15) was from Sigma-Aldrich (Munich, Germany). Anti-phospho-AKT-substrate (23C8D2), used for the detection of phosphorylation of MLH1 at amino acid position serine 477 and hereinafter referred to as anti-p-MLH1, was obtained from Cell Signaling (New England Biolabs GmbH, Frankfurt, Germany). Anti-MLH1 (N-20), anti-CK2α (D-10), and anti-Lamin β (C-20) were from Santa Cruz (Santa Cruz Biotechnology, Heidelberg, Germany). Anti-MLH1 (ab74541) (Abcam, Cambridge UK) and anti-Adaptin γ (clone 88/Adaptin γ (RUO)) were from BD Biosciences (BD Biosciences, Heidelberg, Germany).
Anti-fluorescence-labeled anti-rabbit IRDye800CW and anti-fluorescence-labeled anti-mouse IRDye680LT were from LI-COR (LI-COR Biosciences GmbH, Bad Homburg, Germany).
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2

Immunoblotting for Phospho-MLH1 Detection

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Anti-MLH1 (G168-728) and anti-PMS2 (A16-4) were purchased from Pharmingen (BD Biosciences, Heidelberg, Germany). Anti-beta Actin (Clone AC-15) was from Sigma-Aldrich (Munich, Germany). Anti-phospho-AKT-substrate (23C8D2), used for the detection of phosphorylation of MLH1 at amino acid position serine 477 and hereinafter referred to as anti-p-MLH1, was obtained from Cell Signaling (New England Biolabs GmbH, Frankfurt, Germany).
Anti-fluorescence-labeled anti-mouse IRDye680LT and anti-fluorescence-labeled anti-rabbit IRDye800CW were from LI-COR (LI-COR Biosciences GmbH, Bad Homburg, Germany).
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