Cellular proteins were separated in 10% SDS–polyacrylamide gels, electroblotted onto an Immobilon-P transfer PVDF membrane (Millipore, Temecula, CA, USA), detected with a rabbit anti-human SENP1 mAb (04-453, 1:1000; Millipore), a rabbit anti-Ki67 polyclonal antibody (AB9260, 1:500; Millipore) or a rabbit anti-human-GAPDH antibody (PLA0125, 1:2000; Sigma) and then visualized by a commercial ECL kit (Beyotime, Beijing, China).
Pla0125
Manufactured by Merck Group
Sourced in China
PLA0125 is a laboratory equipment product manufactured by Merck Group. It is a precision instrument designed for various scientific applications. The core function of PLA0125 is to provide accurate and reliable measurements, but a detailed description of its intended use cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab9260, by Merck Group (1 mentions)
Immobilon p pvdf transfer membrane, by Merck Group (1 mentions)
Ecl kit, by Beyotime (1 mentions)
Bolt 4 to 12 bis tris plus, by Thermo Fisher Scientific (1 mentions)
Alkaline phosphatase conjugated anti rabbit igg, by Merck Group (1 mentions)
T per, by Thermo Fisher Scientific (1 mentions)
2 protocols using pla0125
1
Western Blot Protein Detection
2
Western Blot Analysis of P3H3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A kidney was extracted from WT, heterozygous, and null P3H3 mouse and homogenized using T-PER (Thermo Fisher Scientific) containing protease inhibitors at 4 °C. After centrifugation, soluble proteins in the extract were mixed with NuPAGE LDS sample buffer with reducing agents. The protein solutions were separated by Bolt 4 to 12% Bis-Tris Plus (Thermo Fisher Scientific) gel electrophoresis and electrotransferred onto PVDF membranes. The membranes were blocked in PBS solution containing 5% (w/v) skim milk for antibody staining. All proteins were detected by alkaline phosphatase developed with 5-bromo-4-chloro-3-indolyl phosphate and Nitro blue tetrazolium. Rabbit polyclonal antibody against P3H3 (LEPREL2) and rabbit polyclonal antibody against GAPDH were purchased from Proteintech (16023-1-AP) and Sigma-Aldrich (PLA0125), respectively. Alkaline phosphatase-conjugated anti-rabbit IgG (A9919: Sigma-Aldrich) was used as a secondary antibody. Western blotting was performed three times using three independently prepared kidneys.
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