Lipidomics analysis was accomplished as previously described (43 (link)) with minor modifications. Triplicate samples were harvested at serial time points by scraping the bacteria into 25 ml 1:2 (vol/vol) chloroform/methanol, followed by 1:1 (vol/vol) and 2:1 (vol/vol) chloroform/methanol to sterilize samples and extract lipids. The combined extracts were dried, weighed, and redissolved in the starting mobile phase (7:3 hexane/isopropanol) to make a total lipid concentration of 1.0 mg/ml. HPLC-MS was performed using a binary gradient (Agilent 1260) at a flow rate of 0.150 ml/min with detection using a time of flight mass spectrometer (Agilent 6530 Quadrupole Time-of-Flight [QTOF]) and electrospray ionization. Both positive-mode ionization and negative-mode ionization were used in separate analytic runs. Data were analyzed using Mass Hunter (Agilent), the R package XCMS for lipidomic peak analyses (82 (link)), and the R package limma for contrast-based analysis of significance (77 (link)).
6530 quadrupole time of flight qtof
Manufactured by Agilent Technologies
Sourced in United States
The 6530 Quadrupole Time-of-Flight (QTOF) is a high-performance mass spectrometry instrument designed for analytical applications. It combines a quadrupole mass analyzer with a time-of-flight (TOF) mass analyzer, providing high mass accuracy and resolution. The core function of the 6530 QTOF is to accurately measure the mass-to-charge ratio of ionized analytes in a sample.
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Lab products found in correlation
2 protocols using 6530 quadrupole time of flight qtof
1
Lipidomics Analysis of Bacterial Samples
2
Fingerprinting of B. leachii Secretion
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The ngerprinting of B. leachii secretion aqueous extract was performed using the Agilent (Santa Clara, CA, USA) 1260 In nity high performance liquid chromatography (HPLC) system coupled to Agilent 6530 Quadrupole Time of Flight (Q-TOF). The analysis was perfumed using Agilent SB-C18 column (
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