Anti inos antibody 610431

Primary antibodies against GSK3α/β, β-actin, and phosphor-Akt^Ser743 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Antibodies against phosphor-AMPK^Thr172 and phosphor-GSK3α/β^Ser21/Ser9 were purchased from Cell Signaling Technology. Anti-iNOS antibody (610431) was acquired from BD Transduction Laboratories (Lexington, KY, USA). Cyclooxygenase (COX)-2 polyclonal antibody (aa 570–598) was purchased from Cayman Chemicals (Ann Arbor, MI, USA). Heme oxygenase (HO)-1 polyclonal antibody was obtained from Enzo Life Sciences Inc. (Farmingdale, NY, USA). Antibodies against glyceraldehyde-3-phosphate dehydrogenase (GAPDH; GCLC, GCLM, and NQO1) were acquired from Abcam (Cambridge, MA, USA).

IFNγ and TNFα were purchased from R&D Systems. AICAR and metformin were obtained from Toronto Research Chemicals (TRC) and Sigma‐Aldrich, respectively. A‐769662 and Compound C were obtained from TOCRIS Bioscience. Antibodies against pThr172‐AMPKα (#2535), AMPKα (#2603), pSer79‐ACC (#3661), ACC (#3662), pThr389‐pS6K (#9205), S6K (#2708), pSer235/236‐S6 (#2211), and S6 (#2317) were obtained from Cell Signaling Technology. Anti‐iNOS antibody (#610431) was obtained from BD Transduction Laboratories. The anti‐tubulin antibody (DSHB Hybridoma Product 6G7; deposited by Halfter, W.M.) and the anti‐myosin heavy‐chain antibody (DSHB Hybridoma Product MF20; deposited by Fischman, D.A.) were obtained from the Developmental Studies Hybridoma Bank, created by the NICHD of the NIH and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242. Anti‐myoglobin antibody (ab77232) was obtained from Abcam.