The study involved the use of analytical grade solvents which were purchased from Sigma (Sigma-Aldrich USA) i.e. n-hexane (n-Hex); Chloroform (CHCl3); Acetone (Ace); Ethyl acetate (EtOAc); Ethanol ((EtOH); MEthanol (MeOH) and Dimethyl sulfoxide (DMSO). The reagents; Gallic acid (G7385); Quercetin (Q4951); 2,2-diphenyl-1-picryl-hydrazyl (Cat#757621; DPPH); Potassium acetate; Aluminum Chloride; Folin-ciocaltau (F-C) reagent -Sodium carbonate; Ascorbic acid (A5960); Ammonium molybdate; Sodium phosphate; Sulfuric acid; Ferric cyanide; Trichloroacetic acid and Potassium ferricyanide were procured from Merck (Merck KGaA, Germany). Roxithromycin (R4393); Cefixime (1097658); Terbinafine (T8826), Doxorubicin (D1515), Taxol (T1912) and Amphotericin-B (A2942) was obtained from Sigma (Sigma-Aldrich USA) were used Reference standards in the current study.
N hexane n hex
Manufactured by Merck Group
Sourced in United States, Germany
N-hexane (n-Hex) is a colorless, volatile liquid that is commonly used as a laboratory solvent. It has a distinctive odor and is insoluble in water. N-hexane is a saturated aliphatic hydrocarbon with the chemical formula C6H14. It is often used in the extraction and purification of various organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
Acetone, by Merck Group (4 mentions)
Isooctane, by Merck Group (4 mentions)
Dichloromethane dcm, by Merck Group (3 mentions)
Cefixime, by Merck Group (2 mentions)
Doxorubicin, by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Quercetin, by Merck Group (2 mentions)
Terbinafine, by Merck Group (1 mentions)
Potassium acetate, by Merck Group (1 mentions)
Roxithromycin, by Merck Group (1 mentions)
Ferric cyanide, by Merck Group (1 mentions)
Chloroform, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Ethyl acetate, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Trichloroacetic acid, by Merck Group (1 mentions)
Sulfuric acid, by Merck Group (1 mentions)
Potassium ferricyanide, by Merck Group (1 mentions)
6 protocols using n hexane n hex
1
Analytical Evaluation of Phytochemicals
2
PAHs Quantification in Household Dust
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In this study we analyzed the occurrence of the following EPA priority list PAHs in household dust samples; acenaphthylene (Ace), anthracene (Ant), benz[a]anthracene (BaA), benzo[a]pyrene (BaP), benzo[b]fluoranthene (BbF), benzo[g,h,i]perylene (BghiP), benzo[k]fluoranthene (BkF), chrysene (Chr), dibenz[a,h] anthracene (DahA), fluorene (Flu), indeno(1,2,3-cd)pyrene (IcdP), phenanthrene (Phe), and pyrene (Pyr). Following internal standards (ISs) acenaphthylene-D10 (Ace-D10), phenanthrene-D10 (Phe-D10), and chrysene-D12 (Chr-D12) were used for the quantitative analysis. Analytical standards with >99% purity were purchased from Sigma Aldrich (Bellefonte, Pennsylvania (PA), USA). All stock solutions for the analytical standards were prepared in iso-octane, and toluene of different concentrations ranging between 0.1 and 10 µg/mL. For all internal standards, 5 µg/mL were prepared in iso-octane. Silica BondElut (500 mg, 3 mL) cartridges, acetone, dichloromethane (DCM), n-hexane (n-Hex), and iso-octane were of analytical grade obtained from Sigma Aldrich. All glassware used in the sample preparation were baked at 400 °C overnight and kept at 100 °C until use.
3
Analytical Standards for Phthalates and OPEs
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Analytical standards of phthalates namely bis(2-ethylhexyl) phthalate (DEHP), benzyl butyl phthalate (BzBP), bis(2-ethylhexyl) adipate (DEHA), dibutyl phthalate (DBP), diethyl phthalate (DEP), dimethyl phthalate (DMP) and OPEs namely tris (1,3-chloro-2-propyl) phosphate (TCPP), tris (2-chloroethyl) phosphate (TCEP), tris (1,3-dichloro-2-propyl) phosphate (TDCPP) and triphenyl phosphate (TPhP) were purchased from AccuStandards and Sigma Aldrich. Corresponding deuterated (d4-labelled) phthalates were used as internal standards (ISs) for phthalates while TCEP-d12 was used for OPEs. All stock solutions for the analytical standards were prepared in iso-octane and toluene. Acetone, dichloromethane (DCM), n-hexane (n-Hex) and iso-octane were of analytical grade obtained from Sigma Aldrich.
4
Quantification of Organic Pollutants
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Tris (1-chloro-2-propyl) phosphate (TCPP), Tris (2-chloroethyl) phosphate (TCEP), Tris (1, 3-dichloro-2-propyl) phosphate (TDCPP), and Triphenyl phosphate (TPhP) were bought from AccuStandards as analytical standards for OPFRs. Analytical standards for PAHs and phthalates were purchased from Sigma Aldrich in a mixture named EPA-525 semi volatiles and quanti ed using internal standards (i) acenaphthylene (Acy-d10), phenanthrene (Phe-d12), and chrysene (Cry-d12). PAHs namely acenaphthylene (Acy), anthracene (Ant), benz (a) anthracene (BaA), benzo (a) pyrene (BaP), benzo (b) uoranthene (BbF), benzo (g,h,i) perylene (BghiP), benzo (k) uoranthene (BkF), chrysene (Chr), dibenz (a, h) anthracene (DahA), uoranthene (Flu), indeno (1,2,3-cd) pyrene (IcdP), phenanthrene (Phe), and pyrene (Pyr) were analyzed along with benzyl butyl phthalate (BzBP), bis (2-ethylhexyl) adipate (DEHA), bis (2-ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), diethyl phthalate (DEP), and dimethyl phthalate (DMP). Acetone, dichloromethane (DCM), n-hexane (n-Hex), and iso-octane were of analytical grade and obtained from Sigma Aldrich. All the glass used was baked at 400°C overnight and kept at 100°C until use.
5
Antimicrobial and Cytotoxicity Evaluation
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Methanol (MeOH), ethyl acetate (E.A), n-hexane (n-Hex), quercetin, gallic acid, Folin-Ciocalteu (FC) reagent, and sea salt were procured from Sigma-Aldrich (Germany). Reference antibiotics (Ciprofloxacin and Cefixime) were purchased from Sigma-Aldrich. Doxorubicin, nutrient agar, nutrient broth and fetal bovine serum were acquired from Merck (Germany). All chemicals were bought from Sigma-Aldrich unless stated otherwise. Four resistant bacterial strains were used to evaluate the antimicrobial potential of extracts, which include Resistant Streptococcus haemolyticus (R.S. h.; MIC-101), Resistant Escherichia coli (R.E. coli; MIC-102), Resistant Pseudomonas aeruginosa (R.P. a.; MIC-103), and Methicillin-Resistant Staphylococcus aureus (MRSA; MIC-104). Artemia salina (Ocean 90, USA) and freshly isolated human red blood cells (RBCs) were used for cytotoxicity and hemolytic assays.
6
Analytical Standards for NBFRs and PBDEs
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Analytical standards of NBFRs, namely 2-Ethylhexyl-2,3,4,5-tetrabromobenzoate (TBB), Bis (2-ethylhexyl)-3,4,5,6-tetrabromophthalate (TBPH), 1,2-Bis (2,4,6-tribromophenoxy)ethane (BTBPE), and polybrominated diphenyl ethers (PBDEs) (28, 47, 99, 100, 153, 154, 183, and 209 were purchased from AccuStandards and Sigma Aldrich. BDE 77, 128, and labelled BDE 209 were used as internal standards (ISs). All stock solutions for the analytical standards were prepared in iso-octane and toluene. Acetone, dichloromethane (DCM), n-hexane (n-Hex), and iso-octane were of analytical grade obtained from Sigma Aldrich.
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