Human albumin

Manufactured by Fujifilm

Sourced in Japan

Human albumin is a laboratory product derived from human blood plasma. It serves as a critical component in various medical and diagnostic applications, primarily functioning as a protein supplement and stabilizer.

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Lab products found in correlation

B27 supplement, by Thermo Fisher Scientific (4 mentions) Exendin 4, by Merck Group (2 mentions) Activin a, by Merck Group (2 mentions) Rcp μ piece, by Fujifilm (2 mentions) N2 supplement, by Thermo Fisher Scientific (2 mentions) Dmem f12, by Thermo Fisher Scientific (2 mentions) Dulbecco s modified eagle s medium f12, by Thermo Fisher Scientific (1 mentions) Nicotinamide, by Merck Group (1 mentions) Human hepatocyte growth factor, by Funakoshi (1 mentions) Artemisinin, by Merck Group (1 mentions) Carbaryl, by Fujifilm (1 mentions) 1 naphthol, by Fujifilm (1 mentions) Eserine, by Merck Group (1 mentions) Menadione, by Fujifilm (1 mentions)

3 protocols using human albumin

Adipose-Derived Stem Cell Differentiation

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ADSCs (StemPro® Human Adipose-Derived Stem Cells, R7788-115) from Invitrogen (Waltham, MA, USA) were cultured in accordance with the manufacturer’s guidelines. After thawing using established procedures, cells were cultured until passage 5–6, and then 5 × 10⁵ cells were seeded into 96-well ultra-low attachment plates as 3D culture or 12-well ultra-low attachment plates as conventional culture (Sigma-Aldrich Japan Co., LLC., Tokyo, Japan). For Step 1 (Fig. 1A), cells were cultured for 7 days in a differentiation cocktail of DMEM/F12 (Thermo Fisher Scientific Inc., Waltham, MA, USA), 1% human albumin (Wako, Osaka, Japan), 1% B27 supplement (Thermo Fisher Scientific), 1% N2 supplement (Thermo Fisher Scientific), 50 ng/ml activin A (Sigma-Aldrich), 10 nM exendin-4 (Sigma-Aldrich) and with (as 3D culture) or without (as conventional 2D culture) 0.1 mg/mL RCP μ-piece (FUJI FILM, Tokyo, Japan).

Ikemoto T., Feng R., Iwahashi S.I., Yamada S., Saito Y., Morine Y., Imura S., Matsuhisa M, & Shimada M. (2019). In vitro and in vivo effects of insulin-producing cells generated by xeno-antigen free 3D culture with RCP piece. Scientific Reports, 9, 10759.

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Insulin-Producing Cells via 3D Culture

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Insulin-producing cells were generated by 3-dimensional culture using our 2-step protocol. [7] [8] [9] [10] Briefly, 5 × 10 5 ADSCs were seeded into 96-well ultralow attachment plates as a 3-dimensional culture (Sigma-Aldrich Japan Co., LLC., Tokyo, Japan). In Step 1, the cells were cultured for 7 days in a differentiation cocktail of Dulbecco's modified Eagle's medium/F12 (Thermo Fisher Scientific Inc., Waltham, Mass), 1% human albumin (Wako, Osaka, Japan), 1% B27 supplement (Thermo Fisher Scientific), 1% N2 supplement (Thermo Fisher Scientific), 50 ng/mL activin A (Sigma-Aldrich), 10 nM exendin-4 (Sigma-Aldrich), and 0.1 mg/mL RCP μ-piece (Fujifilm, Tokyo, Japan). The cells were then cultured for 14 days in differentiation cocktail, plus 50 ng/mL human hepatocyte growth factor (Funakoshi Co., Ltd), 10 mM nicotinamide (Sigma-Aldrich), and histone deacetylase (HDAC) inhibitor (Sigma-Aldrich) (Fig. 1A).

Ikemoto T., Tokuda K., Wada Y., Gao L., Miyazaki K., Yamada S., Saito Y., Imura S., Morine Y, & Shimada M. (2020). Adipose Tissue From Type 1 Diabetes Mellitus Patients Can Be Used to Generate Insulin-Producing Cells. Pancreas, 49(9).

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Analytical Workflow for Xenobiotic Metabolites

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Methiocarb, carbaryl, 1-naphthol, methiocarb sulfoxide, methiocarb sulfone, 4-methylthio-3,5-xylenol (MX), 3,5-dimethyl-4-(methylsulfinyl)phenol (SP), 3,5-dimethyl-4-(methylsulfonyl)phenol (SOP), menadione, 2-hydroxypyrimidine, rat albumin and human albumin were purchased from Wako Pure Chemical Industries (Osaka, Japan). Eserine, 2-chloro-3,4-dimethoxybenzyl (CDMB), bis(4-nitrophenyl)phosphate (BNPP), 5-pregnen-3β-ol-20-one-16α-carbonitrile (PCN), artemisinin, and bezafibrate (BZF) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Infinity pure dimethyl sulfoxide (DMSO; > 99.5% pure) was purchased from Wako Pure Chemical Industries. Human recombinant CYP and FMO isoforms expressed in a baculovirus system and pooled human plasma were purchased from BD Gentest (Woburn, MA, USA).

Fujino C., Tamura Y., Tange S., Nakajima H., Sanoh S., Watanabe Y., Uramaru N., Kojima H., Yoshinari K., Ohta S, & Kitamura S. (2016). Metabolism of methiocarb and carbaryl by rat and human livers and plasma, and effect on their PXR, CAR and PPARα activities. The Journal of toxicological sciences, 41(5).

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