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Ffpe extracted genomic dna sample preparation

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The FFPE-extracted genomic DNA sample preparation is a laboratory equipment designed to extract and prepare genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. This equipment facilitates the extraction and purification of DNA from FFPE samples, which can be used in various downstream genomic analysis applications.

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Lab products found in correlation

Truseq dna pcr, by Illumina (2 mentions) Truseq nano dna library prep, by Illumina (2 mentions) Hiseq 2500, by Illumina (2 mentions)

2 protocols using ffpe extracted genomic dna sample preparation

1

TruSeq DNA PCR-Free library preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
TruSeq DNA PCR-Free libraries were prepared from blood and FF tissues using 1 μg of input DNA according to the manufacturer’s instructions (Illumina, San Diego, CA). FFPE specimens were prepared using the Illumina FFPE-extracted genomic DNA sample preparation and TruSeq Nano DNA Library Prep (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions (Supplementary Methods). Sequencing was performed on a HiSeq2500 (Illumina, San Diego, CA, USA) to an average depth of coverage of 70 × for tumor samples and 30 × for GL samples. Alignment is detailed in the Supplementary Methods.
Robbe P., Popitsch N., Knight S.J., Antoniou P., Becq J., He M., Kanapin A., Samsonova A., Vavoulis D.V., Ross M.T., Kingsbury Z., Cabes M., Ramos S.D., Page S., Dreau H., Ridout K., Jones L.J., Tuff-Lacey A., Henderson S., Mason J., Buffa F.M., Verrill C., Maldonado-Perez D., Roxanis I., Collantes E., Browning L., Dhar S., Damato S., Davies S., Caulfield M., Bentley D.R., Taylor J.C., Turnbull C, & Schuh A. (2018). Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes Project. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1196-1205.
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2

TruSeq DNA PCR-Free library preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
TruSeq DNA PCR-Free libraries were prepared from blood and FF tissues using 1 μg of input DNA according to the manufacturer’s instructions (Illumina, San Diego, CA). FFPE specimens were prepared using the Illumina FFPE-extracted genomic DNA sample preparation and TruSeq Nano DNA Library Prep (Illumina, San Diego, CA, USA), according to the manufacturer’s instructions (Supplementary Methods). Sequencing was performed on a HiSeq2500 (Illumina, San Diego, CA, USA) to an average depth of coverage of 70 × for tumor samples and 30 × for GL samples. Alignment is detailed in the Supplementary Methods.
Robbe P., Popitsch N., Knight S.J., Antoniou P., Becq J., He M., Kanapin A., Samsonova A., Vavoulis D.V., Ross M.T., Kingsbury Z., Cabes M., Ramos S.D., Page S., Dreau H., Ridout K., Jones L.J., Tuff-Lacey A., Henderson S., Mason J., Buffa F.M., Verrill C., Maldonado-Perez D., Roxanis I., Collantes E., Browning L., Dhar S., Damato S., Davies S., Caulfield M., Bentley D.R., Taylor J.C., Turnbull C, & Schuh A. (2018). Clinical whole-genome sequencing from routine formalin-fixed, paraffin-embedded specimens: pilot study for the 100,000 Genomes Project. Genetics in medicine : official journal of the American College of Medical Genetics, 20(10), 1196-1205.
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