Anti ha antibody h3663

Manufactured by Merck Group

The Anti-HA antibody (H3663) is a monoclonal antibody that specifically recognizes the hemagglutinin (HA) epitope tag. It is a widely used tool in molecular biology and biochemical research for the detection and purification of HA-tagged proteins.

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Lab products found in correlation

Mg132 a2585, by Apexbio (1 mentions) Transit x2, by Mirus Bio (1 mentions) Anti β actin antibody sc 47778, by Santa Cruz Biotechnology (1 mentions) Anti p24 antibody ab9071, by Abcam (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Protease inhibitor cocktail set 3, by Merck Group (1 mentions)

2 protocols using anti ha antibody h3663

Analyzing STOP1 Protein Levels in Arabidopsis Roots

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Twelve-old-day seedlings of Col-0 WT, rae2, and rae3 harboring pSTOP1:STOP1-HA transgene were pretreated with a 0.5mM CaCl₂ solution at pH 4.8 for 6 h and then treated with the same solution containing 0 or 30 μM Al at pH 4.8 for 12 h. Total proteins were extracted from the roots or shoots of each line using a extraction buffer composed of 20 mM Tris–HCl (pH 7.4), 300 mM NaCl, 5 mM MgCl₂ (pH 8.0), 5 mM DTT, 0.5% NP-40, 50 μM MG132 (A2585; APExBIO, United States), and 1× Complete Protease inhibitor tablets EDTA-free (5892791001; Roche). Immunoblot analysis was performed to compare STOP1-HA protein level using anti-HA antibody (H3663; Sigma-Aldrich).

Zhu Y.F., Guo J., Zhang Y, & Huang C.F. (2021). The THO/TREX Complex Component RAE2/TEX1 Is Involved in the Regulation of Aluminum Resistance and Low Phosphate Response in Arabidopsis. Frontiers in Plant Science, 12, 698443.

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APOBEC3 Protein Expression in HEK293T Cells

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HEK293T cells were transfected with pCAG-GS-A3Dv1, v2, v6, v7 or A3B using TransIT-X2 (Mirus, Madison, WI). After 48 hours of incubation, cells were lysed in cell lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 mM NaF, protease inhibitor cocktail set III (EMD Millipore, Darmstadt, Germany) and 1 mM PMSF). Protein lysates (20 μg) were loaded on 10% SDS-polyacrylamide gels and transferred on PVDF membranes (Millipore, Bedford, MA). Anti-HA antibody (H3663) (Sigma-Aldrich, St. Louis, MO), anti- β-Actin antibody (sc-47778) (Santa-Cruz, Santa Cruz, CA) and Anti-p24 antibody (ab9071) (Abcam, Cambridge, UK) were used at 1:10000, 1:500 and 1:1000 dilutions, respectively.

Takei H., Fukuda H., Pan G., Yamazaki H., Matsumoto T., Kazuma Y., Fujii M., Nakayama S., Kobayashi I.S., Shindo K., Yamashita R., Shirakawa K., Takaori-Kondo A, & Kobayashi S.S. (2020). Alternative splicing of APOBEC3D generates functional diversity and its role as a DNA mutator. International journal of hematology, 112(3), 395-408.

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