Pursuit pfp column

Manufactured by Agilent Technologies

Sourced in United States

The Pursuit PFP column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features a pentafluorophenyl (PFP) stationary phase, which provides unique selectivity and retention characteristics for various analytes.

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Lab products found in correlation

Zorbax sb c18 column, by Agilent Technologies (1 mentions) Sodium acetate, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Ethyl acetate, by Merck Group (1 mentions) Epitestosterone, by Merck Group (1 mentions) Ammonium carbonate, by Merck Group (1 mentions) Testosterone 2 3 4 13c3, by Merck Group (1 mentions)

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Pursuit PFP (2 citations)

2 protocols using pursuit pfp column

HPLC-MS/MS Analytical Protocol

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Samples were analyzed by HPLC MS/MS using two different chromatographic methods. The LC System (Agilent 1200 series) comprised a pump model BinPump SL G1312B, a degasser G1379B and an autosampler HiP ALS SL+ G1312B. A Pursuit PFP column (150 mm x 4.6 mm, particle size 3 µm) (method 1) and a Zorbax SB C18 column (100 mm x 2.1 mm, particle size 3 µm) (method 2) were used for the chromatographic separation (Agilent Technologies, Santa Clara, USA). The mobile phase consisted of an ammonium formate buffer (5 mM) containing 0.065% formic acid (pH 3.2) (A) and methanol with 0.2% formic acid (B).

Volpp L., Ferianec V., Ježovičová M., Ďuračková Z., Scherf-Clavel O, & Högger P. (2020). Constituents and Metabolites of a French Oak Wood Extract (Robuvit®) in Serum and Blood Cell Samples of Women Undergoing Hysterectomy. Frontiers in Pharmacology, 11, 74.

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Chiral Separation of Testosterone Metabolites

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Acetonitrile, water of UHPLC/MS grade, formic acid of GC/MS grade, sodium acetate, ammonium carbonate, testosterone-2,3,4-¹³C₃ (Cerilliant analytical reference standards) and epitestosterone were from Sigma-Aldrich (St. Louis, MO). Ethyl acetate and hexane of LC-MS grade were from EMD Millipore Corporation (Billerica, MA). Testosterone, 11ß-MNT and 11ß-MNT-d₆ were provided by the NICHD (Rockville, MD). Normal and charcoal-striped cynomolgus monkey serum were from BioChemed Services (Winchester, VA). A chiral column, Astec CYCLOBOND I 2000 column (5 μm, 50 × 2.1 mm), was from Supelco (Bellefonte, PA). A Pursuit PFP column (3 μm, 100 × 4.6 mm) was from Agilent Technologies (Santa Clara, CA).

Tang L., Swezey R.R., Green C.E., Lee M.S., Bunin D.I, & Parman T. (2022). A Tandem Liquid Chromatography and Tandem Mass Spectrometry (LC/LC-MS/MS) Technique to Separate and Quantify Steroid Isomers 11β-Methyl-19-Nortestosterone and Testosterone. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1193, 123165.

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