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Blue sybrgreen qpcr mastermix

Manufactured by Biozym
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Sourced in Germany

The Blue SybrGreen qPCR Mastermix is a ready-to-use solution for performing quantitative real-time PCR (qPCR) reactions. It contains all the necessary components for amplification and detection of DNA targets, including a proprietary DNA polymerase, dNTPs, and SYBR Green I dye for fluorescent detection.

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Lab products found in correlation

High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (4 mentions)

2 protocols using blue sybrgreen qpcr mastermix

1

Reverse Transcription and qPCR Amplification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolated RNA was reverse transcribed to cDNA by using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, United States), before cDNA was amplified using Blue SybrGreen qPCR Mastermix (Biozym Scientific, Olendorf, Germany). Primer sequences are given in Supplementary Table 1.
Reinisch I., Michenthaler H., Sulaj A., Moyschewitz E., Krstic J., Galhuber M., Xu R., Riahi Z., Wang T., Vujic N., Amor M., Zenezini Chiozzi R., Wabitsch M., Kolb D., Georgiadi A., Glawitsch L., Heitzer E., Schulz T.J., Schupp M., Sun W., Dong H., Ghosh A., Hoffmann A., Kratky D., Hinte L.C., von Meyenn F., Heck A.J., Blüher M., Herzig S., Wolfrum C, & Prokesch A. (2024). Adipocyte p53 coordinates the response to intermittent fasting by regulating adipose tissue immune cell landscape. Nature Communications, 15, 1391.
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2

qPCR Analysis of Gene Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
For qPCR analysis, isolated total RNA was reverse transcribed to cDNA by using High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific, Waltham, MA, United States, 4368814) and amplified using Blue SybrGreen qPCR mastermix (Biozym Scientific, Olendorf, Germany, 331416XL). All primer sequences are listed in Table 1. TFIIb was used as reference gene.
Reinisch I., Klymiuk I., Michenthaler H., Moyschewitz E., Galhuber M., Krstic J., Domingo M., Zhang F., Karbiener M., Vujić N., Kratky D., Schreiber R., Schupp M., Lenihan-Geels G., Schulz T.J., Malli R., Madl T, & Prokesch A. (2022). p53 Regulates a miRNA-Fructose Transporter Axis in Brown Adipose Tissue Under Fasting. Frontiers in Genetics, 13, 913030.
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