Erk 1 2

Manufactured by Bio-Rad

Sourced in United States

Erk-1/2 is a lab equipment product used for the detection and quantification of extracellular signal-regulated kinases 1 and 2 (Erk1/2), which are key components of the mitogen-activated protein kinase (MAPK) signaling pathway. The product allows for the measurement of Erk1/2 activation levels in various cellular samples.

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Lab products found in correlation

P erk 1 2, by Bio-Rad (1 mentions) Mouse and rabbit horseradish peroxidase conjugated igg, by Bio-Rad (1 mentions) P akt, by Bio-Rad (1 mentions) β actin, by Merck Group (1 mentions) Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Bradford assay, by Bio-Rad (1 mentions) Gel logic 1500 imaging system, by Kodak (1 mentions)

2 protocols using erk 1 2

Protein Expression Profiling by Immunoblot

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Total MMEC protein lysates were quantified with the Bradford assay (Bio-Rad, Hercules, CA, USA) and subjected to immunoblot with primary and secondary antibodies to the following: cMet (cod.#8198), phospho (p)-cMet (cod.#3077), VEGFR2 (cod.#9698), p-VEGFR2 (cod.#3770), p-extracellular signal regulated kinase (p-Erk)-1/2 (cod.#4370), Erk-1/2 (cod.#9102), p-Protein kinase B (p-AKT) (cod.#4060), AKT (cod.#9272), p-Phosphoinositide-specific phospholipase C (p-PLC)γ1 (cod.#8713), PLCγ1(cod.#5690), p-P38 mitogen-activated protein kinases (p-p38) (cod.#4511), p38 (cod. #8690) (Cell Signaling Technology, Danvers, MA, USA); beta-actin (Sigma-Aldrich) (cod. A1978); and mouse and rabbit horseradish peroxidase–conjugated IgG (Bio-Rad). Immunoreactive bands were visualized by enhanced chemiluminescence (SuperSignal West Femto Maximum Sensitivity Substrate, Thermo Scientific, Waltham, MA, USA) and the Gel Logic 1500 Imaging System (Eastman Kodak Co.), quantified with the Kodak Molecular Imaging Software, and expressed as arbitrary optical density (OD).

Rao L., De Veirman K., Giannico D., Saltarella I., Desantis V., Frassanito M.A., Solimando A.G., Ribatti D., Prete M., Harstrick A., Fiedler U., De Raeve H., Racanelli V., Vanderkerken K, & Vacca A. (2018). Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin® protein MP0250: a preclinical study. Oncotarget, 9(17), 13366-13381.

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Multiplexed Cytokine and Signaling Profiling

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ELISA kits for mouse IL-27(p28) and IL-10 were from R&D Systems. The IL-27(p28) ELISA has <5% cross-reactivity with rmIL-27(EBI3/p28) and a detection limit of ∼10 pg/ml. Bead-based immunoassays were used for detections of multiple mediators (Bioplex Pro™ mouse cytokine bead-based immunoassay, BioRad, USA) in plasma or phosphorylated signaling proteins (Akt (Ser473), c-Jun (Ser63), CREB (Ser133), ERK1/2 (Thr202/Tyr204, Thr185/Tyr187), JNK (Thr183/Tyr185), MEK1 (Ser217/Ser221), NFκB (Ser536), p38 MAPK (Thr180/Tyr182), STAT3 (Ser727), all from BioRad). Quantification was performed on the Luminex xMAP™/Bioplex-200 System with Bioplex Manager™ Software 5.0.

Bosmann M., Russkamp N.F., Strobl B., Roewe J., Balouzian L., Pache F., Radsak M.P., van Rooijen N., Zetoune F.S., Sarma J.V., Núñez G., Müller M., Murray P.J, & Ward P.A. (2014). Interruption of macrophage-derived IL-27(p28) production by IL-10 during sepsis requires STAT3 but not SOCS3. Journal of immunology (Baltimore, Md. : 1950), 193(11), 5668-5677.

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