Elemental composition was measured using Energy-dispersive X-ray spectroscopy (EDS) in a Philips XL30 ESEM with an Oxford Instruments SiLi detector. The working distance was 12 mm, and approximately 200,000 total counts were taken per point measurement. A total of 2,789 spectra were collected from 66 calcifications in 31 specimens. Composition was calculated in AZtec 2.1 (Oxford Instruments). Measurement of composition in this type of specimen presents several challenges, which must be addressed in order to obtain reproducible results.
Sili detector
Manufactured by Oxford Instruments
Sourced in Germany
The SiLi detector is a solid-state detector designed for X-ray spectroscopy applications. It utilizes a silicon-lithium drifted semiconductor material to detect and analyze the energy of incident X-ray photons.
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Lab products found in correlation
2 protocols using sili detector
1
Elemental Composition of Calcifications
2
Elemental Analysis of Murine Enamel
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Fully mineralized lower hemi-jaws were dissected from perfused adult (approx. 3 months of age) K14-Bcl9/9l-flox, K14-Bcl9/9l-∆HD2 and K14-Pygo1/2-flox, and the respective Bcl9/9lflox, Bcl9/9l-∆HD2 and Pygo1/2-flox controls. Soft tissues were removed manually. The lower jaws were then dehydrated and embedded in Technovit 7200 VLC (Heraeus Kulzer, Wehrheim, Germany). Light-polymerized blocks were mounted on aluminium stubs, polished and coated with a 10-15 nm thick layer of carbon. Thereafter, they were examined using a Tescan EGATS5316 XMSEM (Tescan, Brno, Czech Republic) operated in BSE mode. Elemental composition of enamel was analysed with the aid of energy-dispersive X-ray spectroscopy (EDS). A Si(Li) detector (Oxford Instruments, Wiesbaden, Germany) served for recording EDS spectra using an accelerating voltage of 7 kV, a working distance of 23 mm, and a counting time of 100 s. For the quantitative analysis of these spectra, the Inca energy software (Oxford Instruments) was used.
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