Relative expression of the selected genes was tested using reverse Transcription System (TaKaRa, Dalian, China) and SYBR Green qPCR Mixes (TaKaRa, Dalian, China). The reactions were performed on a 7900 HT Sequence Detection System (Applied Biosystems, USA), under the following procedure: 50 °C for 2 min; 95 °C for 10 min; 95 °C for 15 s, and 60 °C for 1 min with 40 cycles. Relative quantification of the gene expression level was presented using the comparative Ct method (2-ΔCt). The primers used are presented below:
Sybr green qpcr mixes
Manufactured by Takara Bio
Sourced in China
SYBR Green qPCR Mixes are optimized solutions for quantitative real-time PCR (qPCR) reactions. They contain SYBR Green I dye, which binds to double-stranded DNA, enabling detection and quantification of target DNA sequences.
Automatically generated - may contain errors
2 protocols using sybr green qpcr mixes
1
Gene Expression Analysis via qPCR
2
Quantifying Gene Expression Using qPCR
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Total RNA of treated cells was extracted using TRIzol reagent (Invitrogen, Carlsbad, CA). The reverse transcription system (TaKaRa, Dalian, China) and SYBR Green qPCR Mixes (TaKaRa) were used to measure gene expression. The reactions were performed on a 7900HT Sequence Detection System (Applied Biosystems, USA). Relative gene expression level was analyzed using the comparative Ct method (2−ΔCt). The primers used are as follows:
c-Myc: primer F 5′ ATCCTGTCCGTCCAAGCA 3′, primer R 5′ CGCACAAGAGTTCCGTAG 3′
GLS1: primer F 5′ CTGTGCTCCATTGAAGTG 3′, primer R 5′ TGCCCTGAGAAGTCATAC 3′
SLC1A5: primer F 5′ ATCCATGGGCTCCTGGTACT 3′, primer R 5′ CACGCACTTCATCATCAGCG 3′
GAPDH: primer F 5′ AATCCCATCACCATCTTC 3′, primer R 5′ AGGCTGTTGTCATACTTC 3′
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