Complex 2

Manufactured by Abcam

Sourced in United States

Complex II, also known as succinate dehydrogenase (SDH), is a key enzyme complex in the electron transport chain of cellular respiration. It catalyzes the oxidation of succinate to fumarate, coupled with the reduction of ubiquinone to ubiquinol, playing a crucial role in energy production within mitochondria.

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Lab products found in correlation

Complex 1, by Abcam (1 mentions) Tnf α, by Mesoscale (1 mentions) Enhanced chemiluminescence reagent, by GE Healthcare (1 mentions) Gradient sds page gel, by Thermo Fisher Scientific (1 mentions) Ripa buffer, by Boston BioProducts (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Lamp1, by Developmental Studies Hybridoma Bank (1 mentions)

Close spelling variants of this product

MitoTox™ Complex II+ III OXPHOS Activity Assay Kit Complex II Activity Assay Kit Complex II Enzyme Activity Assay Kit Complex II Enzyme Activity Microplate Assay Kit

2 protocols using complex 2

ELISA Quantification of Complex I, II, and TNFα

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Complex I, Complex II (Abcam, Cambridge, MA, USA), and TNFα (Meso Scale Discovery, Rockville, MD, USA) assays were measured by ELISA. All samples were analyzed in duplicate and run in a single assay with intra-assay and percent coefficients of variability of less than 10% for all assays.

Thorwald M.A., Godoy-Lugo J.A., Rodriguez R., Stanhope K.L., Graham J.L., Havel P.J., Forman H.J, & Ortiz R.M. (2022). Cardiac NF-κB Acetylation Increases While Nrf2-Related Gene Expression and Mitochondrial Activity Are Impaired during the Progression of Diabetes in UCD-T2DM Rats. Antioxidants, 11(5), 927.

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Western Blot Analysis of Cellular Proteins

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Standard Western blotting procedures were used. In brief, cells were lysed with ice-cold RIPA buffer (Boston BioProducts) in the presence of 1× protease inhibitor cocktail (Sigma-Aldrich), 1 mM NaF, and 1 mM Na₃VO₄. Total cell lysates were mixed with 2× SDS loading buffer and boiled at 95°C for 10 min. Protein samples (10–100 µg) were then loaded and separated on 4–12% gradient SDS-PAGE gels (Invitrogen) and transferred to PVDF membranes. The membranes were blocked for 1 h with 1% BSA in PBS supplemented with 0.1% Tween20 and incubated with antibodies against GFP (1:10,000; Covance), Lamp1 (1:1,000; Developmental Studies Hybridoma Bank), EEA1 (1:1,000; Santa Cruz Biotechnology, Inc.), and Complex II (1:1,000; Abcam). Bound antibodies were detected using HRP-conjugated anti-rabbit (65-6120) or anti-mouse (62-6520) secondary antibodies (1:5,000; Invitrogen) and enhanced chemiluminescence reagent (GE Healthcare). Band intensities were quantified with ImageJ software.

Wang W., Zhang X., Gao Q., Lawas M., Yu L., Cheng X., Gu M., Sahoo N., Li X., Li P., Ireland S., Meredith A, & Xu H. (2017). A voltage-dependent K+ channel in the lysosome is required for refilling lysosomal Ca2+ stores. The Journal of Cell Biology, 216(6), 1715-1730.

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