Following an overnight fast (8 hours), blood samples were collected. Venous blood was drawn and sera were then collected after centrifugation at 3000×g for 15 min after coagulation. Serum was stored in the refrigerator at 4°C in aliquots until analysis. All laboratory variables were measured by a central laboratory that undergoes regular internal and external quality audits. A fully automatic biochemical analyzer (Roche, Mannheim, Germany) was used to determine the serum parameters. N-terminal pro-B type natriuretic peptide (NT-proBNP) was measured by using an immunoassay based on electrochemiluminescence with the Elecsys 1010/2010 System (Roche Diagnostics, Mannheim, Germany).
Fully automatic biochemical analyzer
Manufactured by Roche
The Fully Automatic Biochemical Analyzer is a laboratory instrument designed for the automated analysis of biochemical samples. It performs a series of assays to measure and quantify various analytes in the samples. The core function of the analyzer is to automate the process of sample handling, reagent addition, incubation, and detection, providing efficient and accurate results.
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Lab products found in correlation
2 protocols using fully automatic biochemical analyzer
1
Serum Biomarker Measurement Protocol
2
Comprehensive Metabolic Profiling in Mice
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Blood glucose was measured through the tail vein every 2 weeks with a One Touch glucometer (Roche). Blood samples were collected by piercing the submaxillary vein monthly. At the end of the study period, the mice were anesthetized with isoflurane and blood samples were collected by eyeball extraction. After centrifugation at 1000 g at 4°C for 15 min, the plasma samples were separated immediately and stored at −80°C until further analysis. Enzyme‐linked immunosorbent assay kits were used to measure insulin (Youersheng, Wuhan, China), glucagon‐like peptide‐1 (GLP‐1; Youersheng), triiodothyronine (T3; Youersheng), and total bile acid (TBA; Youersheng). The homeostasis model assessment‐insulin resistance (HOMA‐IR) was calculated using the formula: HOMA‐IR = FPG (mmol/L) × insulin (mIU/mL) / 22.514 . The levels of total cholesterol (TC), triglyceride (TG), high‐density lipoprotein cholesterol (HDL‐C) and low‐density lipoprotein cholesterol (LDL‐C) were measured with a fully automatic biochemical analyzer (Roche).
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