We used fluorochrome-conjugated antibodies to MSLN (anti-MSLN PE-conjugated Rat IgG2A FAB32652P; R&D Systems), CD3 (PE/Cy7 anti-human CD3 Clone HI3A, 300316; Biolegend), CD45 (FITC Mouse anti-human CD45, 555482; BD Pharmingen), CD4 (APC Mouse Anti-Human CD4, 55349; BD Pharmingen), CD8 (FITC Mouse Anti-Human CD8, 561948; BD Pharmingen), CD62L, CD45RA, and CXCR3 (AF700 mouse anti-human CXCR3, 353742; Biolegend) (Supplementary Table S1 ). In assessing in vitro and in vivo T-cell proliferation and accumulation, cells were labeled with either CFSE or CellTrace Violet cell proliferation stain, in accordance with the manufacturers’ instructions. Cells were quantified using CountBright Absolute Counting Beads (Life Technologies). All flow cytometric analyses were performed on either a BD FACSCalibur or LSR II (BD Biosciences, San Jose, CA); data were analyzed using FlowJo software (version 6.0, TreeStar).
Pe cy7 anti human cd3 clone hi3a
Manufactured by BioLegend
PE/Cy7 anti-human CD3 Clone HI3A is a fluorochrome-conjugated antibody that binds to the CD3 receptor on human T cells. It is used for the identification and enumeration of T cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Facscalibur, by BD (3 mentions)
Countbright absolute counting beads, by Thermo Fisher Scientific (3 mentions)
Anti msln pe conjugated rat igg2a fab32652p, by R&D Systems (3 mentions)
Af700 mouse anti human cxcr3, by BioLegend (3 mentions)
Apc mouse anti human cd4, by BD (1 mentions)
Fitc mouse anti human cd45, by BD (1 mentions)
Fitc mouse anti human cd8, by BD (1 mentions)
3 protocols using pe cy7 anti human cd3 clone hi3a
1
Multiparametric T-cell Phenotyping
2
Multiparametric T-cell Phenotyping
3
Multiparametric T-cell Phenotyping
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We used fluorochrome-conjugated antibodies to MSLN (anti-MSLN PE-conjugated Rat IgG2A FAB32652P; R&D Systems), CD3 (PE/Cy7 anti-human CD3 Clone HI3A, 300316; Biolegend), CD45 (FITC Mouse antihuman CD45, 555482; BD Pharmingen), CD4 (APC Mouse Anti-Human CD4, 55349; BD Pharmingen), CD8 (FITC Mouse Anti-Human CD8, 561948; BD Pharmingen), CD62L, CD45RA, and CXCR3 (AF700 mouse antihuman CXCR3, 353742; Biolegend) (Supplementary Table S1). In assessing in vitro and in vivo T-cell proliferation and accumulation, cells were labeled with either CFSE or CellTrace Violet cell proliferation stain, in accordance with the manufacturers' instructions. Cells were quantified using CountBright Absolute Counting Beads (Life Technologies). All flow cytometric analyses were performed on either a BD FACSCalibur or LSR II (BD Biosciences, San Jose, CA); data were analyzed using FlowJo software (version 6.0, TreeStar).
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