Protein or ip lysate

Manufactured by Beyotime

Sourced in China

Protein or IP lysate is a laboratory product used for the extraction and isolation of proteins from biological samples. It serves as a critical tool for researchers in the fields of biochemistry, molecular biology, and proteomics, enabling the analysis and study of cellular proteins and their interactions.

Automatically generated - may contain errors

Lab products found in correlation

Bca kit, by Boster Bio (2 mentions)

Close spelling variants of this product

Protein lysate (15 citations) IP lysate (8 citations) Lysates (2 citations)

2 protocols using protein or ip lysate

Kidney Tissue Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Kidney tissues and cells were collected using Protein or IP lysate (Beyotime Technology, Shanghai, China). The protein concentration was measured by BCA kit (Boster, Wuhan, China). A total of 10% gradient gels were used. The α-SMA antibody was diluted at 1:3,000, the β-actin antibody at 1:5,000, and the others at 1:1,000. Density analysis of the bands was performed using Gel Pro analysis software.

Yang Y., Wang Y., He Z., Liu Y., Chen C., Wang Y., Wang D.W, & Wang H. (2020). Trimetazidine Inhibits Renal Tubular Epithelial Cells to Mesenchymal Transition in Diabetic Rats via Upregulation of Sirt1. Frontiers in Pharmacology, 11, 1136.

+ Open protocol

+ Expand

Protein and IP Lysate Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells and cardiac tissue were extracted using Protein or IP lysate (Beyotime Technology, Shanghai, China) containing 1:100 protease inhibitors and 1:100 phosphatase inhibitors, and the protein concentration was calculated by BCA kit (Boster, Wuhan, China). 10% and 6-12% gradient gels were used. Antibodies were diluted by 1:1000. Gel Pro analysis software was used for quantification.

Nie J., Duan Q., He M., Li X., Wang B., Zhou C., Wu L., Wen Z., Chen C., Wang D.W., Alsina K.M., Wehrens X.H., Wang D.W, & Ni L. (2018). Ranolazine Prevents Pressure Overload-Induced Cardiac Hypertrophy and Heart Failure by Restoring Aberrant Na+ and Ca2+ Handling. Journal of cellular physiology, 234(7), 11587-11601.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!