Flies were rendered germ-free (GF) following the protocol described in49 (link). GF flies were maintained on fly medium supplemented with antibiotics: kanamycin 50μg/mL (Sigma #K1377), ampicillin50μg/mL (Sigma #A0166), tetracyclin 10μg/mL (Sigma #T7660), erythromycin 5μg/mL (Sigma #E5389). Axenicity was confirmed by crushing the flies and plating the lysate on LB Agar (Carl Roth) and MRS Agar (Carl Roth) plates.
E5389
Manufactured by Merck Group
The E5389 is a laboratory equipment product from Merck Group. It is a general-purpose device designed for various laboratory applications. The core function of the E5389 is to facilitate controlled and precise measurements or analyses in a laboratory setting. No further details about the intended use or specific applications of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Merck Group (1 mentions)
Kanamycin, by Merck Group (1 mentions)
Erythromycin, by Merck Group (1 mentions)
Tetracyclin, by Merck Group (1 mentions)
A0166, by Merck Group (1 mentions)
Lb agar, by Carl Roth (1 mentions)
T7660, by Merck Group (1 mentions)
Mrs agar, by Carl Roth (1 mentions)
C57bl 6 mice, by GemPharmatech (1 mentions)
2 protocols using e5389
1
Germ-Free Fly Maintenance Protocol
2
Osteomyelitis Model in USP8-Deficient Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
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In this study, USP8-deficient (USP -/-) mice with C57BL6 background and wide-type (WT) C57BL6 mice (5~8 weeks old) were obtained from the GemPharmatech (Nanjing, China).
The animals were maintained in a specific pathogen-free (SPF) facility with free access to water and diet. All animal procedures were according to the guidelines of the Animal Care and Use Committee of Shanghai Jiao Tong University Affiliated Sixth People's Hospital (DWLL2018-0093).
A mouse model of osteomyelitis was constructed as previously described (19) . S. aureus (strain ATCC12600T, ATCC, Manassas, VA) were cultured in tryptic soy broth with or without erythromycin (10 μg/mL, E5389, Sigma, St. Louis, MO). The mice were then intramuscularly injected with 2 μl of the bacterial suspension. Bodyweight was monitored and recorded every two days. After 16 days, the mice were utilized and liver and femur tissues were collected. Bacterial load was determined in these tissues. In addition, cortical bone loss
The animals were maintained in a specific pathogen-free (SPF) facility with free access to water and diet. All animal procedures were according to the guidelines of the Animal Care and Use Committee of Shanghai Jiao Tong University Affiliated Sixth People's Hospital (DWLL2018-0093).
A mouse model of osteomyelitis was constructed as previously described (19) . S. aureus (strain ATCC12600T, ATCC, Manassas, VA) were cultured in tryptic soy broth with or without erythromycin (10 μg/mL, E5389, Sigma, St. Louis, MO). The mice were then intramuscularly injected with 2 μl of the bacterial suspension. Bodyweight was monitored and recorded every two days. After 16 days, the mice were utilized and liver and femur tissues were collected. Bacterial load was determined in these tissues. In addition, cortical bone loss
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