Colorimetric wst 1 cell proliferation assay

The colorimetric WST-1 cell proliferation assay (Roche Applied Science; 11644807001) was performed in 96-well white clear bottom plates (Costar, 3610) in quadruplicate, according to manufacturer's instructions [52 (link), 54 (link)]. Absorbance at 440 nm (reflects number of viable cells) was measured using a BioTek Synergy Mx plate reader. Cytotoxicity assays were performed in triplicpate by incubating 1.0 × 10⁶ cells per well 24-well plate in the presence or absence of drugs in a final volume of 1 ml. Aliquots of cells were harvested at indicated time periods and hemocytometer count using Trypan blue exclusion was used to obtain viable cell counts.

The colorimetric WST-1 cell proliferation assay (Roche Diagnostics GmbH, Manheim, Germany) was performed in 96-well white clear-bottom plates (Costar, 3610) in quadruplicate experiments, according to the manufacturer’s instructions. Absorbance at 440 nm (reflects the number of viable cells) was measured using a BioTek Synergy Mx plate reader.