Restriction enzymes were obtained from New England Biolabs (Ipswich, MA, USA) and Toyobo. The Klenow enzyme, T4 DNA ligase (Ligation Kit Ver2.1), and Agarose-LE Classic Type were provided by TaKaRa. Tween 20 and chloroquine diphosphate were obtained from Nacalai. DEAE-dextran, NP-40, leupeptin and pepstatin A were from Sigma. Oligonucleotides were purchased from Gene Design Inc. (Ibaraki, Osaka, Japan). All other chemicals used were of the highest grade commercially available.
T4 dna ligase ligation kit ver2.1
Manufactured by Takara Bio
T4 DNA ligase (Ligation Kit Ver2.1) is a laboratory product that catalyzes the formation of phosphodiester bonds between adjacent 3'-hydroxyl and 5'-phosphate termini in double-stranded DNA molecules. It is an essential component for DNA ligation experiments.
Automatically generated - may contain errors
Lab products found in correlation
Restriction enzyme, by New England Biolabs (3 mentions)
Klenow enzyme, by Takara Bio (2 mentions)
Pepstatin a, by Merck Group (1 mentions)
Deae dextran, by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Np 40, by Merck Group (1 mentions)
Tween 20, by Nacalai Tesque (1 mentions)
Rnase h, by Takara Bio (1 mentions)
Oligonucleotide, by Thermo Fisher Scientific (1 mentions)
5 full race core set, by Takara Bio (1 mentions)
3 protocols using t4 dna ligase ligation kit ver2.1
1
Molecular Cloning Reagents Compendium
2
Molecular Cloning Protocol Reagents
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Restriction enzymes were obtained from New England Biolabs (Ipswich, MA, USA), Toyobo (Osaka-city, Osaka, Japan), and TaKaRa. The Klenow enzyme, T4 DNA ligase (Ligation Kit Ver.2.1) and Agarose-LE Classic Type were provided by TaKaRa. A GENECLEAN III Kit was obtained from BIO101 (La Jolla, CA, USA). Oligonucleotides were purchased from Gene Design Inc. (Ibaraki, Osaka, Japan). All other chemicals used were of the highest grade commercially available.
3
Molecular Cloning Reagents Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Restriction enzymes were purchased from NEB (MA, USA) and Toyobo (Osaka, Japan). RNaseH, T4 DNA ligase (Ligation Kit Ver.2.1), and a 5'-Full RACE Core Set were obtained from TaKaRa. Oligonucleotides were supplied by Invitrogen. A GENECLEAN III Kit was obtained from BIO101 (CA, USA). All other chemicals used were of the highest grade commercially available.
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