Luminescence plate reader

Manufactured by Bio-Rad

The Luminescence plate reader is a versatile laboratory instrument designed to measure luminescent signals. It is capable of detecting and quantifying light emissions from various biological and chemical assays performed in microplate formats. The core function of this device is to provide accurate and reproducible luminescence measurements, enabling researchers to obtain reliable data for their experiments.

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Lab products found in correlation

Ros glotm h2o2 assay kit, by Promega (2 mentions)

2 protocols using luminescence plate reader

Quantifying Cellular Oxidative Stress

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The level of ROS, H₂O₂ was measured by the ROS-GloTM H₂O₂ Assay kit (Promega). For in vitro experiment, 5000 cells were seeded in a 96-well white plate and incubated in normal culture conditions for 24 h. The cells were treated with H₂O₂ for 18 h under normal culture conditions. To perform the assay, the cells were incubated with 25 µl of H₂O₂ substrate solution each well for 6 hours. Then 100 µl of ROS-Glo^™ Detection Solution was applied into each well and incubated at room temperature for 20 min. The luminescent signal was quantified by a luminescence plate reader (Bio-Rad). For human serum samples, each 100 µl of serum sample was incubated with 25 µl of H₂O₂ substrate solution for 6 h and followed by the same step as done in in vitro experiment. Each experiment was repeated three times.

Ng K.T., Yeung O.W., Lam Y.F., Liu J., Liu H., Pang L., Yang X.X., Zhu J., Zhang W., Lau M.Y., Qiu W.Q., Shiu H.C., Lai M.K., Lo C.M, & Man K. (2021). Glutathione S-transferase A2 promotes hepatocellular carcinoma recurrence after liver transplantation through modulating reactive oxygen species metabolism. Cell Death Discovery, 7, 188.

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Quantifying Cellular H2O2 Levels

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The level of a ROS, H 2 O 2 was measured by the ROS-GloTM H 2 O 2 Assay kit (Promega). For in vitro experiment, 5000 cells were seeded in a 96-well white plate and incubated in normal culture condition for 24 hours. The cells were treated with H2O2 for 18 hours under normal culture condition. To perform the assay, the cells were incubated with 25 ul of H 2 O 2 substrate solution each well for 6 hours. Then 100 ul of ROS-Glo™ Detection Solution was applied into each well and incubated at room temperature for 20 min. The luminescent signal was quanti ed by a luminescence plate reader (Bio-Rad). For human serum samples, each 100 ul of serum sample was incubated with 25 ul of H 2 O 2 substrate solution for 6 hours and followed by same step as done in in vitro experiment.

Ng K., Yeung O.W., Lam Y., Liu J., Liu H., Pang L., Yang X., Zhu J., Zhang W.y., Lau M.Y., Qiu W., Shiu H.C., Lai M.K., Lo C.M., & Man K. (2020). Glutathione S-transferase A2 (GSTA2) Promotes Hepatocellular Carcinoma Recurrence After Liver Transplantation Through Modulating Reactive Oxygen Species Metabolism.

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