Rabbit anti micu1

Manufactured by Merck Group

Rabbit anti-MICU1 is a laboratory reagent used for the detection and analysis of MICU1 protein in various research applications. It is a polyclonal antibody raised in rabbits against the MICU1 protein. The primary function of this product is to serve as a tool for researchers to study the expression, localization, and function of MICU1 in biological samples.

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Anti-MICU1 Rabbit anti-

2 protocols using rabbit anti micu1

Comprehensive Antibody Panel for OXPHOS

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The following antibodies were used in our work: rabbit anti-NDUFB8 (Proteintech, 1479-1-AP); mouse anti-MTCO1 (Abcam, ab14705); mouse anti-total OXPHOS antibody cocktail (Abcam, ab110413); mouse anti-GFP (Roche, 11814460001); rabbit anti-VPS39 (Proteintech, 16219-1-AP and Novus Biologicals, NBP1-76535); mouse anti-KDEL (Millipore, 10C3); mouse anti-TOM40 (Santa Cruz, sc-365467); rabbit anti-TOM20 (Proteintech, 11802-1-AP); rabbit anti-LC3B (Sigma, L7543); rabbit anti-MICU1 (Sigma, PA5-83371); guinea pig anti-p62 (Progen, GP62-C); rabbit anti-pS473-AKT (Cell Signaling, 4060S), rabbit anti-AKT (Cell Signaling, 4685); rabbit anti-pT246-PRAS40 (Cell Signaling, 13175); anti-PRAS40 (Cell Signaling, 2691); rabbit anti-GAPDH (Santa Cruz, sc-25778); mouse anti-actin (Sigma, A5316 and abcam, ab14128); mouse anti-tubulin (Sigma, T6074); rabbit anti-SGPL1 (Atlas Antibodies, HPA021125); rabbit anti-AIF (Cell Signaling, 5318).

Jackson J., Wischhof L., Scifo E., Pellizzer A., Wang Y., Piazzesi A., Gentile D., Siddig S., Stork M., Hopkins C.E., Händler K., Weis J., Roos A., Schultze J.L., Nicotera P., Ehninger D, & Bano D. (2022). SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells. Molecular Metabolism, 61, 101503.

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Western Blot Analysis of Mitochondrial Proteins

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Tissues were lysed in modified RIPA buffer (450 mM NaCl, 1% NP-40, 0.1% SDS, 0.5% Deoxycholic acid (sodium salt), 50 mM Tris pH 7.5, 1x complete protease inhibitor). Total cellular protein (30 μg) was separated on SDS-PAGE gels and transferred to nitrocellulose membrane. After blocking in 5% defatted milk, membranes were incubated in primary antibody at 4°C overnight. The primary antibodies used in this study were: rabbit anti-UCP1 (Abcam, ab10983, 1:40,000 for BAT, 1:2000 for co-IP experiments), rabbit anti-EMRE (Santa Cruz Biotech, sc-86337, 1:300), rabbit anti-MCU (Cell Signaling Technologies, 14997, 1:2000) , rabbit anti-MICU1 (Sigma, HPA037479, 1:1000), rabbit anti-HA (Cell Signaling Technologies, 3724, 1:2000) , mouse anti-SDHB (Santa Cruz Biotech, sc-271548, 1:2000) , mouse anti-FLAG (Abmart, 314375, 1:5000), mouse anti-PDHE1α (Santa Cruz Biotech, sc-377092, 1:5000), rabbit anti-phospho-PDHE1α S293 (Millipore, ABS204, 1:2000), rabbit anti-AKT(pS473) (Cell Signaling Technologies, 9271, 1:2000) , rabbit anti-AKT (Cell Signaling Technologies, 9272, 1:2000) , mouse anti-HSP90α/β (Santa Cruz Biotech, sc13119, 1:10000), mouse anti-NDUFS1 (Abcam, ab22094, 1:10000). The membranes were then incubated in HRP-conjugated secondary antibodies at 37°C for 1 h and visualized using Tanon 5200 Chemiluminescent Imaging System.

Xue K., Wu D., Wang Y., Zhao Y., Shen H., Yao J., Huang X., Li X., Zhou Z., Wang Z, & Qiu Y. (2022). The mitochondrial calcium uniporter engages UCP1 to form a thermoporter that promotes thermogenesis. Cell metabolism, 34(9).

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