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4 protocols using capric

1

Antimicrobial Polymer Film Synthesis

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PVDF-co-HFP Kynarflex® (copolymer poly(vinylidene fluoride)-co-hexafluoropropylene) was purchased from Arkema (Colombes, France) and dissolved in N,N´-dimethylformamide (DMF) (p.a.) from P-LAB (Prague, Czech Republic). The caprylic, capric, and lauric acid, glycidol, and chromium acetate hydroxide were supplied by Sigma-Aldrich (St. Louis, MO, USA). All the chemicals were utilized as received without further purification. Both of the bacterial strains -Gram-positive cocci of Staphylococcus aureus ATCC 25923 and Gramnegative rods of Escherichia coli ATCC 25922 -were obtained from the Czech Collection of Microorganisms (CCM, Brno, Czech Republic).
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2

Enzymatic Synthesis of Lipophilic Antioxidants

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Lipase B from C. antarctica (CAL B) immobilized on acrylic resin, Novozym ® 435, was purchased from Novozymes (Bagsvaerd, Denmark). L-ascorbic acid 99.7 % pure was purchased from Zorka, Šabac, Serbia. Oleic acid (Ph. Eur., NF pure) was purchased from AppliChem, Darmstadt, Germany. Saturated fatty acidsacetic, caproic, capric, myristic, and stearic, were obtained from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany, all 98 % pure. 2,2-Diphenyl-1-picrylhydrazyl radical (DPPH) and ascorbyl palmitate (≥ 99%) were purchased from Sigma-Aldrich Chemie Gmbh, as well. Following solvents were used as reaction media: t-butanol (99 %, Sigma-Aldrich Chemie Gmbh, Steinheim, Germany), acetone (99.5 %, Zorka Pharma, Šabac, Serbia), acetonitrile (p.a., Kemika, Zagreb, Croatia), n-hexane (95-98.5 %, Carlo Erba Reagents, Milano, Italy), and isooctane (99.5 % pure, Centrohem, Stara Pazova, Serbia). Hydranal ® -solvent and Hydranal ® -titrant were purchased from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany. HPLC analyses were carried out with methanol and acetonitrile obtained from JT Baker, SAD, and phosphoric acid purchased from Sigma-Aldrich Chemie Gmbh, Steinheim, Germany, all of HPLC grade. Zeolite molecular sieves (diameter 2-3 mm and 0.4 nm pores) were used as water adsorbents.
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3

Dietary Fatty Acids Impact on Broiler Performance

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In the study, 120 oneday-old male broiler chicks (Ross 308) were used. Four main groups were formed including one control and three trials. In each group there were thirty chicks and each main group was divided into three subgroups with 10 chicks in each. The feeding period lasted for 42 days and basically all groups were given periodically formulated isocaloric-isonitrogenic feed for broiler feeding in ad libitum style. In the diet formulations, the NRC 1994 nutritional recommendation was applied. The basal ration contained corn, soybean meal, wheat by-products and vegetable blend oil. The animals in the control group were fed with basal diets (nonadditive added) and the experimental groups' diets were supplemented with 0.2% caprylic (W279900, Sigma-Aldrich, Germany), capric (W236403, Sigma-Aldrich, Germany) and lauric (W261408, Sigma-Aldrich, Germany) acids as free fatty acids respectively.
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4

Formulation of Gelatin-Based Films

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Gelatin type A, wheat gluten, glycerol and sorbitol were obtained from Leiner Davis Gelatin, Rhodia, Synth and Getec, respectively (Brazilian suppliers). Caproic, caprylic, capric, lauric, myristic and palmitic fatty acids were obtained from Sigma-Aldrich (Brazil).
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