6890n gas chromatography 5973 msd mass spectrometer

GC-MS was performed using an Agilent 6890N gas chromatography-5973 MSD mass spectrometer. The samples were analyzed using a fused-silica capillary column, i.e., HP-5MS (5％ phenyl 95％ polydimethylsiloxane, 30 m× 0.25 mm i.d., film thickness＝0.25 μm) and DB-WAX (polyethylene glycol, 15 m×0.25 mm i.d., film thickness＝0.25 μm) . The oven temperature was programmed to increase from 40 to 260℃ at a rate of 4℃/min and then held at 260℃ for 5 min. The flow rate of the carrier gas (He) was 1.5 mL/min. On the DB-WAX column, the oven temperature was programmed to increase from 40 to 240℃ at a rate of 4℃/min and then held at 240℃ for 5 min. The injector and transfer line were held at 230℃, and the ionization energy was 70 eV. The mass range was 39-450 amu. Each sample (1 μL) was injected at a split ratio of 1:40.

An Agilent 6890N gas chromatography-5973 MSD mass spectrometer was used for the GC-MS analysis. The samples were analyzed using a fused-silica capillary column, HP-5MS (5％ phenyl 95％ polydimethylsiloxane, 30 m×0.25 mm i.d., film thickness＝0.25 μm) and DB-WAX (polyethylene glycol, 15 m×0.25 mm i.d., film thickness＝ 0.25 μm) . The oven temperature was programmed as follows: increase 40 to 260℃ at the rate of 4℃/min, with a held at 260℃ for 5 min. The flow rate of the carrier gas (He) was 1.5 mL/min. On the DB-WAX column, the oven temperature was increased from 40℃ to 240℃ at the rate of 4℃/ min and held at 240℃ for 5 min. The injector and transfer line temperatures were 230℃, and the ionization energy was 70 eV. The mass range was 39-450 amu. One μL of the sample was injected, and the split ratio maintained was 1:40.