Axiophot hb0 50

Myofibroblasts were identified by staining with anti-alpha smooth muscle actin (a-SMA) monoclonal antibody (mouse clone 1A4; Dako, Glostrup, Demark). The secondary antibody was Texas red goat anti-mouse IgG (Molecular Probes). Nuclei were stained with DAPI (Molecular Probes). Limbal blood vessels were used as positive controls, and omission of the primary antibody provided negative controls. Immunofluorescence sections (from TUNEL, BrdU, and a-SMA) were examined under an Axiophot fluorescence-incorporated microscope (Zeiss Axiophot HB0-50; Carl Zeiss, Oberkochen, Germany) and photomicrographs were captured using the AxioCam HRc Digital Camera and Axiovision release 4.8 software (Carl Zeiss).

Myofibroblast cells were identified by staining with anti-α-SMA monoclonal antibody (mouse clone 1A4, Dako, Glostrup, Demark). The secondary antibody was Texas red goat antimouse IgG (Molecular Probes). Sections were examined under an Axiophot fluorescence-incorporated microscope (Zeiss Axiophot HB0-50, Carl Zeiss, Germany) and photomicrographs were captured using the AxioCam HRc Digital Camera and Axiovision release 4.8 software (Carl Zeiss, German). The wall of the limbal blood vessels served as positive control, and omission of the primary antibody provided negative controls.
TUNEL, BrdU and α-SMA positive cells were quantitatively counted in an area of 10 000 µm 2 of two slides of each cornea at 10× magnification using the Touch Count function in the deep stroma where the segment was implanted.

). The secondary antibody was Texas red goat anti-mouse IgG (Molecular Probes). Sections were examined under an Axiophot fluorescence-incorporated microscope (Zeiss Axiophot HB0-50, Carl Zeiss, Germany) and photomicrographs were captured using an AxioCam HRc digital camera and Axiovision release 4.8 software (Carl Zeiss). Limbal blood vessels served as positive controls, and omission of the primary antibody provided negative controls.