X rad 320

Manufactured by Hamilton Company

The X-RAD 320 is a versatile x-ray generating device capable of producing high-energy x-rays for a variety of laboratory applications. It features a stable x-ray tube and precise controls for adjusting the voltage and current, enabling users to generate x-rays with customizable energy and intensity levels.

Automatically generated - may contain errors

Lab products found in correlation

22 gauge needle, by Hamilton Company (2 mentions) Collagenase d, by Roche (1 mentions) Lymphocyte separation medium, by MP Biomedicals (1 mentions) Anti human cd34 microbeads, by Miltenyi Biotec (1 mentions)

2 protocols using x rad 320

Humanized Mouse Model via Fetal Liver CD34+ Cells

Cited 2 times

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Recipient mice were engrafted with human hematopoietic progenitor cells as previously described (14 (link), 71 (link)). Briefly, human fetal liver samples were cut into small fragments and treated with collagenase D (Roche) 100 ng/mL for 45 min at 37 °C. Human CD34⁺ cells from the resulting cell suspension were purified from the fetal liver by density gradient centrifugation (Lymphocyte Separation Medium, MP Biomedicals) followed by positive immunomagnetic selection with the EasySepTM Human CD34 Positive Selection Kit (StemCell). Cells were frozen in FBS (fetal bovine serum) containing 10% dimethyl sulfoxide (DMSO) and stored in liquid nitrogen. For intrahepatic engraftment, newborn 1 to 3-d-old pups were irradiated with 80 rad using a cabinet irradiator (X-RAD 320) and then injected with 10,000 fetal liver CD34⁺ cells in PBS into the liver with a 22-gauge needle (Hamilton Company).

Odell I.D., Agrawal K., Sefik E., Odell A.V., Caves E., Kirkiles-Smith N.C., Horsley V., Hinchcliff M., Pober J.S., Kluger Y, & Flavell R.A. (2023). IL-6 trans-signaling in a humanized mouse model of scleroderma. Proceedings of the National Academy of Sciences of the United States of America, 120(37), e2306965120.

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Engrafting Human CD34+ Cells in NSG Mice

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Human CD34⁺ cells were purified from fetal liver samples by density gradient centrifugation followed by positive immunomagnetic selection with anti-human CD34 microbeads (Miltenyi Biotech). Newborn NSG mice (within first 3 days of life) were sublethally irradiated (x-ray irradiation with X-RAD 320 irradiator at 180 cGy), and 100,000 to 150,000 CD34⁺ cells in 20 µl of phosphate-buffered saline were injected into the liver with a 22-gauge needle (Hamilton Company).Mice were used for experiments 10 to 12 weeks after transplantation. NSG mice treated with the PTPN22 inhibitor were injected with the PTPN22 inhibitor 0.75 or 0.15 mg ip twice daily for a week. All animals were treated, and experiments were conducted in accordance with the Yale institutional reviewed guidelines on treatment of experimental animals.

Schickel J.N., Kuhny M., Baldo A., Bannock J.M., Massad C., Wang H., Katz N., Oe T., Menard L., Soulas-Sprauel P., Strowig T., Flavell R, & Meffre E. (2016). PTPN22 inhibition resets defective human central B cell tolerance. Science immunology, 1(1), aaf7153.

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