Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS) were obtained from Gibco ® , and antibiotic and antimycotic solutions were obtained from Caisson ® . The cervical carcinoma cell line (HeLa, ATCC®-CCL2™) was obtained from the American Type Culture Collection (ATCC). Secundiflorol G (purity of 93%) was isolated from Aeschynomene fascicularis in a previous study with (Caamal-Fuentes et al., 2015) . Additional inputs included annexin V-FITC/IP (Miltenyi Biotec); caspases 9, 8, and 3/7 Glo-Assay Kit and Diamond Acid Dye (Promega); Quick-g DNA Miniprep extraction Kit (Zymo Research); 2,7-dichloromethyldihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen™); and rhodamine 123 (Sigma Aldrich).
Quick g dna miniprep extraction kit
Manufactured by Zymo Research
Sourced in United States
The Quick-g DNA Miniprep extraction Kit is a laboratory tool designed to quickly and efficiently extract genomic DNA from a variety of sample types. The kit utilizes a simple and streamlined protocol to purify high-quality DNA suitable for downstream applications such as PCR, sequencing, and other molecular biology techniques.
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Lab products found in correlation
3 protocols using quick g dna miniprep extraction kit
1
Investigating Secundiflorol G's Anticancer Effects
2
Nematode Egg Extraction and DNA Isolation
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Egg extraction was done according to Carneiro et al. (2004) , and second-stage juveniles (J2) were extracted from egg masses, handpicked and placed on a modified Baermann funnel for hatching.
Total genomic DNA was extracted from c. 200 to 300 lL of nematode eggs using a regular phenol-chloroform extraction method as described by Randig et al. (2002) . Genomic DNA was also extracted from single J2 using the Quick gDNA Mini-Prep extraction kit (Zymo Research) according to the manufacturer's instructions.
Total genomic DNA was extracted from c. 200 to 300 lL of nematode eggs using a regular phenol-chloroform extraction method as described by Randig et al. (2002) . Genomic DNA was also extracted from single J2 using the Quick gDNA Mini-Prep extraction kit (Zymo Research) according to the manufacturer's instructions.
3
Whole Blood DNA Extraction Protocol
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Extraction of DNA was carried out on whole blood using the Quick-gDNA Mini Prep extraction kit (Zymo Research, USA) using the recommended manufacturer's protocol. Briefly, 400 µl of genomic lysis buffer was added to 100 µl of blood, vortexed, and allowed to settle at room temperature for 5–10 minutes. The solution was then transferred into a Zymo-Spin Column in a collection tube and centrifuged for a minute. The columns were then washed with DNA prewash buffer (200 µl) followed by a wash buffer (500 µl). Afterwards, 90 µl of elution buffer was added to the column to elute the DNA. The eluted genomic DNA (gDNA) was subsequently quantified using a nanodrop 2000C and either used immediately or stored at −20°C.
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