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11 protocols using sodium phosphate dibasic

1

Analytical Grade Chemicals Protocol

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Unless specified, all chemicals used for analytical purposes were analytical grade. Alcalase® (protease from Bacillus licheniformis), β-mercaptoethanol, D-L-Leucine, sodium tetraborate and o-phtaldialdehyde (OPA reagent) were purchased from Sigma Aldrich (St Louis, Missouri, MO, USA). Hexane, hydrochloric acid, methanol and sodium hydroxide were purchased from Fisher Scientific (Ottawa, ON, Canada). Citric Acid monohydrate, hydrochloric acid (36.5–38% v/v), NaCl, sodium hydroxide pellets and sodium phosphate dibasic were purchased from VWR International (Mississauga, ON, Canada). Sodium dodecyl sulfate (SDS) was purchased from Bio Basic (Markham, ON, Canada). Food grade canola oil was purchased from a local grocery store in Quebec City.
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2

Antioxidant Capacity Quantification Assays

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ABTS (2,2′-azinobis(3-ethylbenzothiazoline 6-sulfonic acid)), Trolox® (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), DTT (dl-dithiothreitol), diatomaceous earth (Hyflo® Super Cel®), Trizma® base, fluorescein sodium salt, and formic acid (reagent grade ≥ 95%) were purchased from Sigma-Aldrich (Steinheim, Germany). AAPH (2,2′-azobis(2-amidinopropane)) (Acros Organics), Tris (ultrapure), potassium dihydrogen phosphate, potassium sulfate, sodium phosphate dibasic, sodium chloride, ortho-boric acid, glycine (proteomics grade), and methanol (HPLC–MS grade) were provided by VWR International Eurolab S.L. (Barcelona, Spain). Glacial acetic acid, sulfuric acid, and sodium hydroxide were supplied by Fisher Scientific (Madrid, Spain). SDS (sodium dodecyl sulfate, purissimum-CODEX) and nitric acid (65% p/p) were obtained from Panreac (Barcelona, Spain). Acetonitrile (HPLC grade), acetone, glycerol, and bromophenol blue indicator (ACS reagent) were supplied by Merck (Darmstadt, Germany). Octadecyl C18 sorbent was from Phenomenex (Madrid, Spain), while absolute ethanol was from J.T. Baker (Deventer, The Netherlands). Anhydrous magnesium sulfate (99.5% min powder) was provided by Alfa Aesar (Karlsruhe, Germany). Deionized water (resistivity >18 MΩ/cm) was obtained through a Milli-Q SP® Reagent Water System (Millipore Corporation Bedford, Bedford, MA, USA).
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3

Pharmacological Protein Purification Protocol

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Sodium chloride (NaCl), potassium chloride (KCl), sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), and acetonitrile were all purchased from VWR International (Ville Mont-Royal, QC, Canada). Anti-polyethylene glycol antibody [PEG-B-47] was obtained from Abcam (Toronto, ON, Canada). SureBeads magnetic beads with protein A were bought from Biorad Laboratories (Mississauga, ON, Canada). Tween-20 was purchased from Fisher Scientific (Ottawa, ON, Canada). Flurbiprofen and ketorolac were obtained from Cayman Chemical Company (Ann Harbour, MI, USA).
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4

Enzymatic Assay of Test Molecules

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NADPH, NADH, Ethyl 4-chloro acetoacetate, DMSO were purchased from Sigma-Aldrich, USA, Sodium Phosphate monobasic, Sodium Phosphate dibasic, Sodium Chloride, Imidazole were purchased from Amresco, USA and was the finest grade available. All bacterial growth media and Isopropyl-β-d-thiogalactoside (IPTG) were obtained from HiMedia, India. Test molecules were procured from eMolecules Library, USA.
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5

Synthesis and Characterization of Metallic Nanoparticles

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The metallic precursors iron (II) chloride (FeCl2), iron (III) chloride (FeCl3), N,N’-Diisopropylcarbodiimide (DIC), 4-dimethylamino-pyridine (DMAP), dimethyl sulfoxide (DMSO), dichloromethane (DCM), dimethylformamide (DMF), Pd black, sodium carbonate (Na2CO3), dextran, xanthine oxidase from bovine milk (XOD) were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3,4,5-tris(benzyloxy)benzoic acid (TBBA) was purchased from Tokyo Chemical Industry (Tokyo, Japan). Ammonium hydroxide (NH4OH, 28-30 wt%) was purchased from BDH (Poole, Dorset, UK). Polyethylene glycol with various molecular weights was purchased from Sigma-Aldrich and Alfa Aesar (Heysham, Lancashire, UK). Phosphotungstic acid hydrate powder was purchased from Alfa Aesar (Heysham, Lancashire, UK). Sodium phosphate monobasic, sodium phosphate dibasic, and potassium hydroxide (KOH) were purchased from Amresco (Solon, OH, USA). Hypoxanthine (HX) was purchased from Research Products International (RPI) Corp. (Mt. Prospect, IL, USA). All chemicals used were of analytical grade.
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6

Immunohistochemical Detection of Glucose Transporters

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We obtained acetone, ethanol, methanol, potassium chloride, potassium phosphate monobasic, sodium chloride, sodium hydroxide, sodium phosphate dibasic, disodium hydrogen arsenate heptahydrate, phosphoric acid (Ultrex II), and xylene from JT Baker (Estado de México, México). Tris–HCl was purchased from Gibco BRL (Rockville, Maryland, USA) and monomethylarsenate from Supelco (St. Louis, Missouri, USA). 3-Aminopropyl triethoxysilane, hydrogen peroxide, paraformaldehyde, polyoxyethylene sorbitan monolaurate (Tween 20), dimethylarsonic acid, sodium arsenite (NaAsO2), and sodium borohydride were purchased from Sigma-Aldrich (St. Louis, Missouri, USA). Entellan mounting resin and sodium hydroxide were obtained from Merck (Darmstadt, Germany). For the immunohistochemistry (IHC) analysis, polyclonal anti-human GLUT1, GLUT3, and GLUT4 antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, California, USA). A Histostain-plus kit and hematoxylin were obtained from Zymed Lab., Inc. (San Francisco, California, USA). Mice were fed pellets of Mouse Diet 5015 fish meal free from LabDiet, Purina Mills, Inc. (Richmond, Indiana, USA).
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7

Preparation and Characterization of Influenza Vaccine

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Two lots of influenza monovalent H1N1 vaccine (H1N1 California/07/2009 X179A) were provided by Novartis Pharmaceuticals (East Hanover, NJ). The first lot contained 79 μg hemagglutinin (HA)/mL, and the second lot contained 406 μg HA/mL. Sucrose, glycine, sodium phosphate dibasic, sodium phosphate monobasic (USP or NF) and polysorbate 80 were obtained from J.T.Baker (Center Valley, PA). Trehalose dehydrate (USP/NF) was purchased from Pfanstiehl, Inc. (Waukegan, IL). Arginine, dextran 40, mannitol, and NaCl (reagent or analytical grade) were purchased from Sigma-Aldrich (St. Louis, MO).
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8

Antioxidant and Anti-Inflammatory Assays

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DPPH, β-carotene, linoleic acid, chloroform, 5-LOX, α-tocopherol, Griess reagent, glycerol mono-oleate, sodium nitroprusside, sodium sulfate, 95% ethanol, Tween20, and Tween40 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, l-ascorbic acid, 99% sodium chloride, and sodium phosphate dibasic were purchased from J.T. Baker (Phillipsburg, NJ, USA). l-Tyrosine and tyrosinase were purchased from Worthington (Lakewood, NJ, USA). 96% α-Bisabolol, 96% citronellal, and 95% citronellol were purchased From Alfa Aesar (Heysham, UK). Modified letheen broth was purchased from Oxoid (Basingstoke, UK). Agar, yeast extract, malt extract, peptone, and malt extract broth were purchased from HiMedia (Mumbai, India). Dextrose anhydrous was purchased from Nihon Shiyaku Reagent (Tokyo, Japan). Deionized distilled water was purified by the Milli-Q system (Millipore, Bedford, MA, USA).
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9

Chitosan-Based Biochemical Assay Protocol

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Chitosan, medium molecular weight, deacetylation value of 75–85%, and viscosity of 200–800 cP, was purchased from Aldrich Chemical Co.
Bovine serum albumin, acrylamide, N,N′-methylenebisacrylamide, trichloroacetic acid (TCA), FeSO4, sulfuric acid, cumene hydroperoxide (CHP), butylhydroxytoluene, methanol, xylenol orange, di-nitrophenylhydrazine (DNPH), guanidine, ethanol, ethyl acetate, hydrochloric acid, Coomassie® Brilliant Blue R-250, thioglycolic acid, NBD-Cl, and o-phthalaldehyde (OPA) were purchased from Sigma-Aldrich (St. Louis, Missouri, USA).
Thiobarbituric acid was purchased (TBA) from Fluka (Sigma-Aldrich, Toluca, MX); sodium chloride, EDTA disodium salt, N,N′,N′- tetramethylethylenediamine (TEMED), Tris (base), urea, β-mercaptoethanol, glycine, acetic acid glacial, sodium phosphate monobasic, sodium phosphate dibasic, and copper sulfate pentahydrate were purchased from J.T. Baker (Pennsylvania, USA); sodium carbonate and lactic acid were purchased from Fermont (Monterrey, MX); sodium dodecyl sulfate (SDS) and bromophenol blue were obtained from Hycel (Mexico, MX); and plate count agar was purchased from Bioxon, Becton and Dickinson (Mexico, MX). All reagents used were of analytical grade.
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10

Electrochemical Sensor Fabrication Protocol

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1,6-hexanedithiol (1,6-HDT), 6-mercapto-1-hexanol (MCH), potassium chloride, potassium phosphate monobasic, and sodium chloride were purchased from Sigma-Aldrich (Burlington, MA, USA). Sodium phosphate dibasic was purchased from J. T. Baker (Phillipsburg, NJ, USA). Potassium ferricyanide and potassium ferrocyanide were purchased from SHOWA (Antarctica, Japan). Distilled deionized water (>18 MΩ) was obtained from ELGA LabWater (High Wycombe, UK).
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